Waters Technologies Corporation

Waters Investments Limited

Martin Gilar

Geoff C Gerhardt

Joseph D Michienzi

Sylvain Gilles Cormier

Pamela Carmen Iraneta

Keith Fadgen

Edouard S P Bouvier

Jeffrey Musacchio

Jason F Hill

Michael Donegan

Thomas S McDonald

Matthew A Lauber

Michael O Fogwill

Bernard Bunner

Raymond P Fisk

Jonathan L Belanger

Abhijit Tarafder

Yuri Tuvim

John Charles Gebler

Ying-Qing Yu

Jennifer Fournier

John E O'Gara

Wade P Leveille

Richard Wayne Andrews

Peyton C Beals

Charles T Murphy

Robert A Jencks

Bruce J Compton

Kerri M Smith

Jordan Kornfeld

Rachel Mumma

Scott Mccall

Joseph A Jarrell

Mathew H DeLano

Christopher C Benevides

Uwe Dieter Neue

Mark Basile

Edouard S Bouvier

Peter Jeng-Jong Lee

Sebastien Besner

Ying Qing Yu

Elizabeth K Brown

Fang Xia

Christopher DesJardins

Armand Gatien Ngounou Wetie

Peter J Lee

Elizabeth K Kane

Thomas Mcdonald

Jennifer H Fournier

The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

Polyanionic acids to improve recovery and minimize system loss

Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.

Insulated serial column chromatography arrangements and systems

Sample preparation and separation can be performed using a sample cartridge (). The cartridge includes a barrel () with a first and second end, a column segment () connected to the second end of the barrel, and a column () containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

Micro solid phase extraction devices and methods

Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge () comprising a barrel () and a column (), and especially including reinforcement to the column permitting high-pressure separation.

The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.

Parallel insulated chromatography columns

Described herein are a selectable flow restriction device and a fluidic network for chromatographic sample dilution during injection. A selectable flow restriction device is used in combination with a splitter to define an adjustable splitter for separating a high-pressure solvent flow into two separate solvent flows having the same solvent composition. The first solvent flow passes to a sample manager where a sample is injected as a discrete fluidic plug at an injection valve into a flow comprising at least one solvent. The second solvent flow is merged downstream from the location of injection with the first solvent carrying the sample fluidic plug. The second solvent flow dilutes the sample plug during the mixture of the two solvent flows.

Sample injection for liquid chromatography using split solvent flow

The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.

Coated flow path components for chromatographic effects

The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.

Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding

An ion detector comprises a surface configured to receive one or more ions and a detector configured to detect one or more ions by detecting electromagnetic radiation scattered by one or more ions at the surface.

Ion detector

Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.

Method for determining a dwell volume of a chromatographic system

Exemplary embodiments are directed to methods and systems for controlling fluid parameters within a detector. A first and second fluid pump manager control the flow of a first and second fluid to one or more heating/cooling devices, and a mixer receives and mixes the first and second fluids. An optical detector flow cell receives the fluid mixture from the mixer, and a pressure regulator controls the pressure at the optical detector flow cell. Thus, the composition, temperature, and pressure of a fluid mixture entering an optical detector flow cell can be controlled in real time.

System and method for monitoring phase-separation and mutual miscibility of fluid mixtures

A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

Method and apparatus for injecting a chromatographic sample

Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.

Methods for focusing analyte peaks in liquid chromatography using a spatial temperature gradient are provided. Also provided are methods for focusing analyte peaks and improving resolution using a trap column upstream of a separation column. Further, methods are provided in which the trap column placed upstream of the separation column is packed with a temperature-sensitive polymer/copolymer, and a spatial temperature gradient is applied along the trap column for obtaining improved retentivity by trap column stationary phase, and overall improved resolution of analyte peaks.

Spatial temperature gradients in liquid chromatography

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Franklin, MA, United States of America

Martin Gilar