Hoechst Aktiengesellschaft

Martin Gerl

Rainer Obermeier

Rupert Timpl

Jurgen Ludwig

Walter Sabel

Cornelia Steinert

Manfred Quint

Monoclonal antibodies for selective immunological determination of high molecular weight, intact laminin forms in body fluids. The present invention relates to monoclonal antibodies for the selective immunological determination of high molecular weight forms of laminin in body fluids, to a process for preparing these antibodies, and to their use for diagnosing diseases. The antibodies according to the invention preferably bind to intact, native laminin, in particular to the structures of the laminin P1 domain of the laminin which are folded in a native manner.

Monoclonal antibodies for selective immunological determination of high

Monoclonal antibodies directed to the laminin P1 domain for selective immunological determination of native, high molecular weight, intact laminins in body fluids; a process for preparing these antibodies; and their use for diagnosing diseases. These antibodies preferably bind to intact, native laminin, in particular to the structures of the laminin P1 domain of laminin which are folded in a native manner.

A process for obtaining insulin having correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan, chaotropic auxiliaries and at a pH of 10 to 11 is described, as well as the direct conversion of the proinsulin to insulin with the aid of enzymes and direct isolation of the insulin from the reaction mixture with the aid of hydrophobic adsorber resins.

Process for obtaining insulin having correctly linked cystine bridges

A process is described for obtaining proinsulin possessing correctly linked cystine bridges from a corresponding proinsulin amino acid chain in the presence of mercaptan and chaotropic auxiliary agents and at a pH of 10 to 11, as well as its direct isolation from the reaction mixture using hydrophobic adsorber resins.

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Kriftel, Germany

Martin Gerl