Glaxosmithkline Biologicals Sa

Janssen Pharmaceuticals, Inc.

Martin Edward Braun

Michael T Kowarik

Stefan Jochen Kemmler

Manuela Mally

Jeroen Geurtsen

Darren Robert Abbanat

Patricia Ibarra Yon

Veronica Gambillara Fonck

Eveline Marleen Weerdenburg

Pieter Jan Burghout

Maria Paula Carranza Sandmeier

Jan Poolman

Jan Theunis Poolman

Kellen Cristhina Fae

Amirreza Faridmoayer

Sabina Marietta Gerber

Markus Mueller

Michael Thomas Kowarik

Fonck Veronica Gambillara

Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate to be produced. The oligosaccharyl transferase enzymes can be PglB oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein to vaccinate a subject against extra-intestinal pathogenic. (ExPEC).

Methods of producing bioconjugates ofO-antigen polysaccharides, compositions thereof, and methods of use thereof

A bioconjugate of anglucosylated O4 antigen polysaccharide covalently linked to a carrier protein and compositions thereof are provided. Also provided are recombinant host cells for producing the bioconjugate, and methods of producing the bioconjugate using the recombinant host cells. The recombinant host cells contain a nucleic acid encoding a glucosyl transferase capable of modifying theO4 antigen with glucose branching to produce the glucosylated O4 antigen polysaccharide. Bioconjugates of anglucosylated O4 antigen polysaccharide described herein can be used alone or in combination with one or more additionalO-antigen polysaccharides to induce antibodies against anglucosylated antigen, and to vaccinate a subject against extra-intestinal pathogenic(ExPEC).

Bioconjugates ofO-antigen polysaccharides, methods of production thereof, and methods of use thereof

Methods of producing bioconjugates ofo-antigen polysaccharides, compositions thereof, and methods of use thereof

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The present invention discloses modifiedHIa proteins which show reduced tendency to aggregate, improving protein stability and yield. Said modified HIa proteins optionally also contain glycosylation site consensus sequences. The invention also discloses a conjugate comprising a modified HIa protein and an antigen (for example asaccharide antigen), wherein the antigen is linked to an amino acid residue of the modified HIa protein.

Immunogenic composition

Growing community of inventors

Cham, Switzerland

Martin Edward Braun