Metanomics Gmbh and Co. Kgaa

Metanomics Gmbh

Basf Plant Science Company Gmbh

Martin Dostler

Tilmann B Walk

Ralf Looser

Michael Manfred Herold

Joachim Kopka

Martin Kluttig

Britta Lehmann

Annette Hennig

Achim Fegert

Silke Heidemann

Regine Fuchs

Maria Wahl

Benjamin Hentschel

Gerd Balcke

Susan Carvalho

Silke Klein

The present invention provides a method for profiling phytohormone levels in plant tissue or tissue of other plastid containing organisms, i.e. a method for the simultaneous determination of a multitude of phytohormone levels in plant tissue or tissue of other plastid containing organisms.

Method for profiling phytohormone levels in plant tissue

The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites including extracting a biological sample with a extraction buffer containing a phase separator and a volatile neutral ammonium salt under conditions which allow for immediate disruption of cells in the biological sample, separating the polar metabolites in the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample containing cellular material is contemplated.

Methods for analyzing polar metabolites of the energy metabolism

The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Process for extracting constituents from organic material

The present invention relates to a process for isolating constituents from organic material, comprising the following process steps:

Method for extraction and analysis of contents made from organic material

A method for characterizing a sample containing at least one compound is disclosed. Initially, a three-dimensional set of data is generated through sample analysis using time resolved and mass resolved separation techniques. The data comprises at least one signal as a function of a mass variable over a first range of measurement and of a time variable over a second range of measurement. The first range of measurement is divided into at least two mass variable intervals, and for each mass variable interval, an extracted signal is selected which is a function of the time variable. The second range of measurement is divided into at least one time variable interval. A characteristic value is then selected for each time variable interval and each extracted signal to generate a characteristic sample profile, comprising one or more characteristic values as functions of respective time variable intervals and respective mass variable intervals.

System and method for characterizing a chemical sample

Method for the extraction of components made from organic material

The invention relates to a method for the extraction of components made from organic material, comprising the step of extraction with a single-phase mixture of 50 to 90 vol. % methanol and 50 to 10 vol. % water and optionally 0 to 40 vol. % of a further solvent or solvent mixture.

Method for the extraction and analysis of contents made from organic material

The invention relates to a mass spectrometry method for analysing mixtures of substances using a triple quadrupole mass spectrometer, whereby said mixtures of substances are ionised prior to analysis. The invention is characterised in that the method comprises the following steps: a) selection of a mass/charge quotient (m/z) of an ion created by ionisation in a first analytical quadrupole (I) of the mass spectrometer; b) fragmentation of the ion selected in step (a) by applying an acceleration voltage in an additional subsequent quadrupole (II), which is filled with a collision gas and acts as a collision chamber; c) selection of a mass/charge quotient of an ion created by the fragmentation process in step (b) in an additional subsequent quadrupole (III), whereby steps (a) to (c) of the method are carried out at least once; and d) analysis of the mass/charge quotients of all the ions present in the mixture of substances as a result of the ionisation process, whereby the quadrupole (II) is filled with collision gas, but no acceleration voltage is applied during the analysis. Steps (a) to (c) and step (d) can also be carried out in reverse order.

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Henningsdorf, Germany

Martin Dostler