Symrise Ag

Leibniz-Institut Fur Pflanzenbiochemie

Leibniz-Institut Für Pflanzenbiochemie Stiftung Des Offentlichen Rechts

Martin Dippe

Ludger A Wessjohann

Jakob Peter Ley

Torsten Geissler

Katrin Geissler

Andrea Porzel

Wolfgang Brandt

Susann Herrmann

Hannes Rost

The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.

Mutant 4-hydroxyphenylacetate 3-hydroxylases and uses thereof

The present invention relates to isolated polypeptides that are derived from wildtypeS-Adenosylmethionine (SAM) synthase or from a biologically active fragment thereof, wherein said isolated polypeptides comprise an amino acid sequence that, in relation to the amino acid sequence of said wildtypeSAM synthase or of the biologically active fragment thereof, comprises at least one amino acid substitution, selected from the group consisting of amino acid substitutions at positions I317 and I105. The present invention further relates to respective isolated nucleic acids, vectors, host cells, uses and methods for the production of SAM derivatives.

S-adenosylmethionine (SAM) synthase variants for the synthesis of artificial cofactors

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Halle, Germany

Martin Dippe