Bayer Aktiengesellschaft

Bayer Healthcare Aktiengesellschaft

Other

Bayer Schering Pharma Ag

Greiner Bio-one Gmbh

Cybio Instruments Gmbh

Bayer Aktiengelsellschaft

Bayer Intellectual Property Gmbh

Martin Bechem

Rainer Gross

Siegbert Hebisch

Siegfried Goldmann

Jurgen Stoltefuss

Alexander Straub

Joachim Hutter

Matthias Schramm

Howard-Paul Rounding

Michael Kayser

Gerhard Franckowiak

Horst Boshagen

Wolfgang Paffhausen

Gunther Thomas

Delf Schmidt

Andreas Schade

Horst Meyer

Gunter Thomas

Eckhard Schwenner

J Urgen Stoltefuss

Thoams Krahn

Joachim H Utter

Albrecht Marhold

Johannes-Peter Stasch

Elisabeth Perzborn

Klemens Lustig

Carsten Schmeck

Klaus Dembowsky

Friedel Seuter

Christoph Gerdes

Thomas Krahn

Michael Sperzel

Ulrich Nielsch

Werner Stürmer

Ralph Noll

Claudia Hirth

Ulrich Rosentreter

Thomas Moore

Michael Lögers

J&uuml;rgen Stoltefuss

Heiko Oehme

Fred R Heiker

Jürgen Stoltefuss

Michael L&ouml;gers

Günther Knebel

Wolfgang Kraemer

Gabriele Br&auml;unlich

Georg Schmidt

Werner St&uuml;rmer

Ranier Gross

Siegfried Goldman

Joachim Hu/ Tter

Ju/ Rgen Stoltefuss

Gabriele Bräunlich

Siegfried Hebisch

The present invention relates to an improved microplate which is constructed of at least one frame part and at least one bottom part, where the bottom part has a maximum thickness of 500 &#x3bc;m.

Multiwell microplate with transparent bottom having a thickness less than 200 micrometers

A process for the quantitative optical analysis of fluorescently labeled biological cells involves contacting a cell layer on a transparent support at the bottom of a reaction vessel with a solution containing the fluorescent dye. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved.

Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays

In a process for the quantitative optical analysis of fluorescently labelled biological cells, a cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dye. The sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dye, which absorbs the excitation lightfor the fluorescent dyeand/or its emission light, is added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottom. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatant. Instead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom

Masking background fluorescence and luminescence in the optical analysis of biomedical assays

In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.

In a process for the quantitative optical analysis of fluorescently labelled biological cellsa cell layer on a transparent support at the bottomof a reaction vesselis in contact with a solutioncontaining the fluorescent dyeThe sensitivity of analytical detection can be considerably improved if to the fluorescent dyealready present in addition a masking dyewhich absorbs the excitation lightfor the fluorescent dyeand/or its emission lightis added to the solutionand/or if a separating layerpermeable to the solution and absorbing and/or reflecting the excitation lightor the emission lightis applied to the cell layer at the bottomThis process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layermust in this case be composed such that it has a high power of reflection for the luminescent light. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layerat the bottomof a reaction vesselis in contact with a solution (supernatant) in which a fluorescent or luminescent ligandis dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dyewhich absorbs the excitation lightfor the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatantInstead of the masking dye in the solutionor optionally as an additional measure, a separating layerpermeable to the solutionand absorbing and/or reflecting the excitation lightand/or the emission light or the luminescent light can be applied to the cell or receptor layerat the bottom

Masking background flourescence and luminescence in optical analysis of biomedical assays

The invention relates to a fluorescence-measuring system as can be employed for high-throughput screening in drug development. The arrangement for fluorescence excitation contains a two-dimensionally extended sample-receiving device and at least two illumination sources for exciting the fluorescence of the samples. The illumination sources are extended linearly and arranged in such a way that the illuminated area of the sample-receiving device is homogeneously illuminated directly or via deflecting mirrors at an opening angle of &#x2266;30&#xb0;. A detector system for the fluorescence light from the sample-receiving device is arranged on either side of the sample-receiving device in such a way that it detects fluorescence emission from the area of measurement at an angle outside the range of reflection of the excitation light of the illumination sources at the illuminated area of the sample-receiving device, preferably at an angle in the range from 80&#xb0; to 100&#xb0;, particularly preferably about 90&#xb0;, to the extended plane of the area of the sample-receiving device.

Fluorescence-measuring system

The present invention relates to 6-carboxyphenyldihydropyridazinone derivatives having the formulawherein R, R, R, A, D, G, and E are as defined in the specification and claims, a process for preparing these materials, pharmaceutical compositions containing them, and a method for using them in the treatment of anemias.

6-carboxyphenyldihydropyridazinone derivatives and use thereof

Tetrahydroquinolinyl 6-methyldihydrothiadiazinone derivatives and use thereof

In a process for the quantitative optical analysis of fluorescently labelled biological cells , a cell layer on a transparent support at the bottom of a reaction vessel is in contact with a solution containing the fluorescent dye . The sensitivity of analytical detection can be considerably improved if to the fluorescent dye already present in addition a masking dye , which absorbs the excitation light for the fluorescent dye and/or its emission light , is added to the solution and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to the cell layer at the bottom . The separating layer must be composed such that it has a high power of reflection for the luminescent light . Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer at the bottom of a reaction vessel is in contact with a solution in which a fluorescent or luminescent ligand is dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dye which absorbs the excitation light for the fluorescent dye and/or its emission light or the luminescent light is added to the supernatant . Instead of the masking dye in the solution or optionally as an additional measure, a separating layer permeable to the solution and absorbing and/or reflecting the excitation light and/or the emission light or the luminescent light can be applied to the cell or receptor layer at the bottom

Masking background fluorescence and luminescence in optical analysis of biomedical assays

An arrangement for transporting microtitration plates in an automatic handling machine is disclosed comprising at least one linear-transport device on which a carriage, which has at least one place of deposit for a microtitration plate, is transported in a straight line, under automatic control, between two limit positions. A lifting device is included with vertically displaceable bolts by which the microtitration plates can be lifted off or deposited on the carriage. At least one rotary device is also included in which a rotary element which has at least one resting place for a microtitration plate can be moved, under automatic control, about a fixed rotation shaft. The rotary device is designed and arranged in such a way with respect to the linear-transport device that the resting place can be rotated between the place of deposit and the microtitration plate which has been lifted and is resting on the bolts, so that the surface centers of gravity of the place of deposit of the resting place and of the microtitration plate lie on a line which is parallel to the bolts.

Arrangement for transporting microtitration plates in an automatic handling machine

In the measurement system for detecting optical signals of microassays, the signal-generating test objects,are arranged on an investigation surface of a planar carrier,The planar carrier,is, in particular, a microtitre plate for biological objects. In principle, the measurement system comprises an optical imaging arrangement which reduces the size of the test objects,to be measured in such a way that all the objects are imaged completely on a two-dimensional, photosensitive image sensor,For imaging, a high-resolution glass-fiber taper element,having a large-area,and a small-area end,is used in this case, whose end surfaces,are selected such that the large-area end surface,corresponds at least to the investigation surface of the carrier,and the small-area end surface,corresponds to the size of the image sensor,the ratio of the end surfaces,producing the scale of reduction of the optical imaging arrangement in order to image the investigation surface of the carrier,completely onto the image sensor

Optical measurement system for detecting luminescence or fluorescence signals

The invention relates to new 4-bicyclically substituted dihydropyridines of the general formula (I) ##STR1## in which R.sub.1 to R.sub.5 have the meaning given in the description, processes for their preparation and their use in medicaments, in particular in agents for the treatment of cardiovascular diseases.

4-bicyclically substituted dihydropyridines, and their use in medicaments

This invention relates to 3-quinolyl-substituted dihydropyridine derivatives, such as ##STR1## or a pharmaceutically acceptable salt thereof. These compounds are used in treating cardiovascular disease.

3-quinolyl-substituted dihydropyridines, and their use in medicaments

Light activable 'caged' 1-(2-nitrobenzyl)-substituted

Intermediate of the formula ##STR1## wherein R.sup.5 represents a cyclic, straight-chain or branched, saturated or unsaturated hydrocarbon radical having up to 12 carbon atoms, which is optionally interrupted up to twice in an identical or different manner by oxygen or sulphur, as intermediates for substituents on novel dihydropyridine cardiovascular compounds.

3-quinolyl-substituted dihydropyridines, processes for their preparation

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Wuppertal, Germany

Martin Bechem