Endorecherche, Inc.

Justbio Inc.

Martin Beaulieu

Alain Belanger

Sophie Chabot

Eric Levesque

Dean W Hum

A simple extraction method for preparing an organic certifiableextract with high sweetness and optionally with antioxidant properties is provided. The extraction method involves extraction ofleaves that have been dried and grinded with a hot solvent generally regarded as safe (GRAS), allowing the dissolution of the sweetening compounds naturally present therein. These sweetening compounds include steviosides and rebaudioside A, which are purified from the extracts by two or more successive chromatographic purifications steps. The sweetening compounds are then eluted with an appropriate elution solvent. The simplicity and organic certification of the method provide advantages over the methods currently employed in theindustry.

[object Object]

A uridine diphospho-glucuronosyltransferase (UGTB217) is provided. Methods of producing the enzyme and using the enzyme to identify potential compounds which inhibit or alter the activity of the enzyme are described. In addition, methods of using antibodies to localize the protein, or using the gene sequence or portions thereof for probes or using the gene sequence to produce expression-disrupting sense or antisense DNA fragments thereof, or antisense RNA, are provided.

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CA

Martin Beaulieu