Massachusetts Institute of Technology

The General Hospital Corporation

Martha L Gray

Jose G Venegas

Alan R Shapiro

Luis A Melendez

Jonathan L Schaffer

Mehmet Toner

Martin Arnold Schmidt

Joel Voldman

John D Wright

James K Roberge

Wright John D

Rebecca Braff

A cell analysis and sorting apparatus is capable of monitoring over time the behavior of each cell in a large population of cells. The cell analysis and sorting apparatus contains individually addressable cell locations. Each location is capable of capturing and holding a single cell, and selectively releasing that cell from that particular location. In one aspect of the invention, the cells are captured and held in wells, and released using vapor bubbles as a means of cell actuation. In another aspect of the invention, the cells are captured, held and released using electric field traps.

Cell analysis and sorting apparatus for manipulation of cells

Disclosed is a method for imparting to a living culture of cells biaxial mechanical forces which approximate the mechanical forces to which cells are subject in vivo. The method utilizes a displacement applicator which may be actuated to contact and stretch a membrane having a living cell culture mounted thereon. Stretching of the membrane imparts biaxial mechanical forces to the cells. These forces may be uniformly applied to the cells, or they may be selectively nonuniformly applied.

Cell stretching method

Disclosed is an apparatus and method for imparting to a living culture of cells biaxial mechanical forces which approximate the mechanical forces to which cells are subjected in vivo. The apparatus includes a displacement applicator which may be actuated to contact and stretch a membrane having a living cell culture mounted thereon. Stretching of the membrane imparts biaxial mechanical forces to the cells. These forces may be uniformly applied to the cells, or they may be selectively non-uniformly applied.

Cell stretching apparatus

A technique for measuring capacitance and displacement, in which two circuits are combined, one that produces a current proportional to capacitance, and another that integrates that current between two reference levels, and supplies an output proportional to the integration time. In preferred embodiments, the first circuit is a capacitive pump circuit, and the second circuit a dual-slope integrator.

Method for the measurment of capacitance, with application to linear

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Cambridge, MA, United States of America

Martha L Gray