Baxter International Inc.

Martha C Pattillo

Arnold C Bilstad

David V Bacehowski

T Michael Dennehey

Herbert M Cullis

James W Yang

William C Brown

A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.

Method for metering nutrient media to cell culture containers

System for metering nutrient media to cell culture containers and method

Growing community of inventors

Evanston, IL, United States of America

Martha C Pattillo