Labcyte Inc.

Marsha N Blauwkamp

Richard N Ellson

Richard G Stearns

Babur B Hadimioglu

Sammy S Datwani

Joseph D Olechno

Jennifer M Hardee

Justin Blonigan

Carson Riche

Methods of ejecting droplets containing a non-Newtonian fluid by an acoustic droplet ejector can include applying a tone burst of focused acoustic energy to a fluid reservoir containing a non-Newtonian fluid at sufficient amplitude to effect droplet ejection according to a tone burst pattern. The tone burst pattern may include three discrete tone burst segments, the first tone burst segment having greater duration than the second and third segments, and third segment having greater duration than the second segment. The exact durations and amplitudes of the tone burst segments can be tuned to influence the ejection properties.

Acoustic droplet ejection of non-newtonian fluids

A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

High-efficiency transfection of biological cells using sonoporation

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San Jose, CA, United States of America

Marsha N Blauwkamp