Eastman-kodak Company

Marsha B Oenick

Richard C Sutton

Biologically active reagents are prepared from particles of copolymers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through reactive groups on the particle surface. These reagents are used to advantage in analytical elements and methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents. Adsorption of undesirable proteins on the particles of the reagents was considerably reduced because of the specific composition of the particles.

Method for detection of a nucleic acid using particulate reagent having

Biologically active reactive are prepared from particles of copolymers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through reactive groups on the particle surface. These reagents are used to advantage in analytical elements and methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents. Adsorption of undesirable proteins on the particles of the reagents was considerably reduced because of the specific composition of the particles.

Particulate biologically active reagent containing polyoxyalkylene side

Copolymers are prepared from (a) at least about 0.5 mole % of monomers having reactive groups which are, directly or indirectly, capable of reaction with free amine or sulfhydryl groups of biologically active substances, (b) from about 0.1 to about 20 mole % of monomers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88, and (c) up to about 99.4 mole % of oleophilic monomers which provide hydrophobicity to the copolymers. The copolymers can be supplied as latex particles in aqueous compositions.

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Rochester, NY, United States of America

Marsha B Oenick