10X Genomics, Inc.

Marlon Stoeckius

Christina Galonska

Caroline Julie Gallant

Layla Katiraee

James Michael Chell

Jonathan Alles

Katherine Pfeiffer

Yifeng Yin

Jennifer Chew

Michael Schnall-Levin

Jonas Frisen

Cedric Uytingco

David Sukovich

Felice Alessio Bava

Albert Dale Kim

Joseph Francis Shuga

Octavian Marian Bloju

Aleksandra Jurek

Anna-Maria Katsori

Mesruh Turkekul

Ryo Hatori

Rapolas Spalinskas

Patrick Roelli

Andrej Hartnett

Dulce Ovando Morales

Zachary Bent

Marco Mignardi

Arundhati Shamoni Maheshwari

Kim Thrane

Naishitha Anaparthy

Ariel Royall

Denise Cheung

Alexander Hermes

Alexander Gagnon

Qirong Lin

Wyatt James Mcdonnell

Stefania Giacomello

Jeffrey Mold

Geoffrey P Mcdermott

Camilla Engblom

Erik Leonard Henrik Borgstrom

Michael John Terry Stubbington

Meghan L F Frey

An electrophoretic system is provided for analyte capture from a biological sample. The electrophoretic system can be used to permeabilize the sample to allow analytes to be released from the sample. For example, the sample can be contacted with capture probes attached to a substrate, and an electric field created by the electrophoretic system can cause analytes to be released from the cell, and effectively migrate toward and bind to the capture probes attached to the substrate.

Electrophoretic system and method for analyte capture

Provided herein are methods of identifying abundance and location of an RNA in a biological sample using an adaptor sequence and a primer. Also disclosed herein are kits, compositions, and systems that are used to perform the methods.

Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis

Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

Spatial analysis of epigenetic modifications

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

Methods for spatial analysis using RNA-templated ligation

Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

Multiplex capture of gene and protein expression from a biological sample

Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3′ end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3′ end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.

Methods for identifying a location of an RNA in a biological sample

Provided herein are methods, compositions, and kits for removing a portion of a sequence in a member of a nucleic acid library.

Nucleic acid library methods

Provided herein are methods and systems for analysis of one or more single cells. The disclosed methods may comprise barcoding analytes from cell types of interest, while eliminating undesired cell types from analysis. Barcoding may comprise the use of a targeting agent. A targeting agent may be attached to an inhibitor or an activator, thereby inhibiting or activating analyte barcoding.

Methods and systems for selective analysis of cellular samples

Provided herein are methods of detecting target nucleic acids and uses of the same.

Compositions and methods of making gene expression libraries

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Spatially-tagged analyte capture agents for analyte multiplexing

Provided herein are methods of identifying a location of an RNA in a sample that include: (a) contacting the sample with an array comprising capture probes, where a capture probe comprises a capture domain and a spatial barcode; (b) releasing the RNA from the sample; (c) extending a 3' end of the capture probe using the capture domain-bound RNA as a template; (d) generating nick(s) in the extended capture probe-hybridized RNA and performing random-primed DNA synthesis; (e) performing end repair on the second strand DNA molecule; (f) adding a single adenosine nucleotide to the 3&#x2032; end of the extended capture probe; (g) ligating a double-stranded sequencing adaptor to the double-stranded DNA product; and (h) determining all or a part of the sequence of the RNA, and the sequence of the spatial barcode, or complements thereof, and using the determined sequences to identify the location of the RNA in the sample.

Spatial analysis of DNA methylation

Growing community of inventors

Stockholm, Sweden

Marlon Stoeckius