Koninklijke Philips Corporation N.v.

Biomerieux, Inc.

Siemens Healthineers Nederland B.v.

Marlieke Joan Overdijk

Toon Hendrik Evers

Harma Martine Feitsma

Pieter Jan Van Der Zaag

Anja Van De Stolpe

Matthias Irmscher

Thomas Johannes Van Gijsel

Stefan Johannes Hendrikus Toonen

Willem-Jan Arend De Wijs

Godefridus Johannes Verhoeckx

Bernardus Jozef Maria Beerling

Monica Scholten

Joost Hubert Maas

Wilbert Hendrik Bouwman

Nicole Henrica Maria Smulders

Anja Van De Stople

Peter T G Sillekens

Saskia Van De Laar

Eveline Catharina Anna Clasina Den Biezen-Timmermans

Tom Van Gijsel

A device is provided comprising a receiving chamber for receiving a patient's sample, a first reagent reservoir and a second reagent reservoir. The receiving chamber may be configured for receiving at least an end portion of a sample collecting element, with the patient's sample being collected by that sample collecting element. The first reagent reservoir may generally be configured to accommodate a liquid reagent which may include a lysing agent, i.e. the liquid may be a kind of a lysis buffer. Likewise, the second reagent reservoir may generally be configured to accommodate a dry reagent which may include magnetic particles. The device may further comprise at least one of the following two actuators, a first actuator for supplying a first reagent from the first reagent reservoir to the sample and a second actuator for supplying a second reagent from the second reagent reservoir to the resulting mixture.

System for preparing a sample

In order to provide a system allowing an improved and facilitated preparation of a sample for an appropriate analysis, a device for preparing a patient's sample before analysis is provided. The device comprises a housing () and an actuator (). The housing includes a receiving chamber (). The receiving chamber is configured for receiving a liquid. The actuator is movable relative to the housing. The actuator is configured for extracting a predetermined amount of the liquid received in the receiving chamber and for supplying the extracted liquid to a reagent chamber, so that the reagent is suspended in the liquid. The actuator comprises a metering chamber () configured for receiving the predetermined amount of the liquid and for supplying the predetermined amount of the liquid to the reagent chamber.

System for applying a reagent to a sample

The invention relates to a cartridge () for processing of a liquid sample, for example for the detection of components in a sample of blood. The cartridge comprises a fluidic system with an intake port () leading via an intake capillary channel () to a storage chamber (). Moreover, a feeding capillary channel () leads from the storage chamber () to a detection chamber (). The design of the cartridge () is such that the intake capillary channel () that connects the intake port () to the storage chamber (), has a capillary suction pressure sufficiently high to drive some sample from the intake port to the storage chamber without need of any additional pressure. Furthermore the cartridge a flow control element () adapted to be externally controllable such that the sample can be drawn from the storage chamber () towards the processing chamber () without any active pumping.

Cartridge for fast sample intake

The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridize to capture probe nucleic acids which are immobilized to a support via their 5' end. The hybridization product is further extended with a polymerase to form a double stranded nucleic acid. To this double stranded nucleic acid, a hairpin nucleic acid is ligated. This ligation product is further amplified and sequenced.

Method for amplifying nucleic acids

The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with a novel human coronavirus causing Severe Acute Respiratory Syndrome (SARS). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of SARS nucleic acid. The oligonucleotide sequences provided with the present invention are located in the replicase gene, the nucleocapsid gene and the 3' end non-coding region of the SARS Coronavirus genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of SARS Coronavirus can be obtained. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.

Nucleic acid sequences that can be used as primers and probes in the amplification and detection of SARS coronavirus

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Helmond, Netherlands

Marlieke Joan Overdijk