Expression Pathology Incorporated

Life Technologies Corporation

USA As Represented by Secretary of the Navy

Marlene M Darfler

David B Krizman

Gulilat Gebeyehu

Ayoub Rashtchian

Irina A Nazarenko

Mekbib Astatke

Richard M Pires

Joseph Solus

Bradley R Ringeisen

Peter K Wu

Jason Barron

David B Krisman

Thomas G Guiel

Casimir P Eitner

Methods are provided for directly converting histopathologically processed biological samples, tissues, and cells into a multiuse biomolecule lysate. This method allows for simultaneous extraction, isolation, solubilization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in biochemical assays.

Liquid tissue preparation from histopathologically processed biological samples, tissues and cells

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

Primers and methods for the detection and discrimination of nucleic acids

The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

Liquid tissue preparation from histopathologically processed biologically samples, tissues and cells

The invention provides methods for diagnosing diseases such as cancer and other conditions using biological samples. Liquid Tissue samples prepared from histopathologically prepared tissue obtained from a subject surprisingly can be used to identify and, optionally, to quantify analytes that are diagnostic of the presence of a disease, condition or syndrome in the subject.

Diagnosis of diseases and conditions by analysis of histopathologically processed biological samples using liquid tissue preparations

The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solubilization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

The invention provides methods for multiplex analysis of biological samples of formalin-fixed tissue samples. The invention provides for a method to achieve a multiplexed, multi-staged plurality of Liquid Tissue preparations simultaneously from a single histopathologically processed biological sample, where the protocol for each Liquid Tissue preparation imparts a distinctive set of biochemical effects on biomolecules procured from histopathologically processed biological samples and which when each of the preparations is analyzed can render additive and complementary data about the same histopathologically processed biological sample.

Multiplex liquid tissue&#x2122; method for increased proteomic coverage from histopathologically processed biological samples, tissues and cells

Multiplex method for increased proteomic coverage from histopathologically processed biological samples, tissues cells

A method of laser forward transfer is disclosed. Photo energy is directed through a photon-transparent support and absorbed by an interlayer coated thereon. The energized interlayer causes the transfer of specific regions of a heterogeneous tissue sample coated thereon across a gap and onto a receiving substrate or into a receiving vessel.

Growing community of inventors

Gaithersburg, MD, United States of America

Marlene M Darfler