The University of British Columbia

Markus Stein

Brendan Kenny

B Brett Finlay

Michael Donnenberg

Li-Ching Lai

Rebekah DeVinney

Brett Finlay

The present invention provides a polypeptide, called EspA, which is secreted by pathogenic, such as the enteropathogenic (SPEC) and enterohemorrhagic (EHEC). The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing

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A polypeptide, called Tir (for translocated intimin receptor, which is secreted by attaching and effacing pathogens, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC). These bacterial pathogens inserts their own receptors into mammalian cell surfaces, to which the bacterial pathogen then adheres to trigger additional host signaling events and actin nucleation. Diagnosis of disease caused by pathogeniccan be performed by the use of antibodies which bind to Tir to detect the protein or the use of nucleic acid probes for detection of nucleic acids encoding Tir polypeptide. Isolated nucleic acid sequences encoding Tir polypeptide, Tir peptides, a recombinant method for producing recombinant Tir, antibodies which bind to Tir, and a kit for the detection of Tir-producingare provided. A method of immunizing a host with Tir to induce a protective immune response to Tir or a second polypeptide of interst is also provided. A method for screening for compounds which interfere with the binding of bacterial pathogens to their receptors is further provided.

Host receptor for pathogenic bacteria

A polypeptide, called Tir (for translocated intimin receptor, which is secreted by attaching and effacing pathogens, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC). These bacterial pathogens inserts their own receptors into mammalian cell surfaces, to which the bacterial pathogen then adheres to trigger additional host signaling events and actin nucleation. Diagnosis of disease caused by pathogeniccan be performed by the use of antibodies which bind to Tir to detect the protein or the use of nucleic acid probes for detection of nucleic acids encoding Tir polypeptide. Isolated nucleic acid sequences encoding Tir polypeptide, Tir peptides, a recombinant method for producing recombinant Tir, antibodies which bind to Tir, and a kit for the detection of Tir-producingare provided. A method of immunizing a host with Tir to induce a protective immune response to Tir or a second polypeptide of interest is also provided. A method for screening for compounds which interfere with the binding of bacterial pathogens to their receptors is further provided.

Method for detecting a host receptor for pathogenic bacteria

The present invention provides a polypeptide, called EspA, which is secreted by pathogenic, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC). The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing

The present invention provides the EspA polypeptide, which is secreted by pathogenic such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Diagnosis of disease caused by such pathogenic can be performed by standard techniques, such as those based upon the use of antibodies which bind to EspA to detect the protein, as well as those based on the use of nucleic acid probes for detection of nucleic acids encoding EspA protein. The invention also provides isolated nucleic acid sequences encoding EspA, EspA polypeptide, EspA peptides, a method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing The invention also provides a method of immunizing a host with EspA to induce a protective immune response to EspA.

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Quercegrossa, Italy

Markus Stein