Euroimmun Medizinische Labordiagnostika Ag

Markus Morrin

Martin Rateike

Tilman Johannes Sumpf

Martin Hagen-Eggert

Helge Pannhoff

Christian Piepke

Matthias Muller

Thomas Laudan

Matthias Müller

Michael Falkert

Michael Pape

Kristin Rentzsch

Winfried Stocker

Matthias MÜLLER

A method and microscopy system are useful for recording an image of a sample region. A laser beam is directed onto the sample region with interface(s). An objective lens facilitates images the laser beam on a focusing point which lies on the optical axis of the objective lens or an axis parallel thereto, and which lies in a focusing plane. The objective lens and the sample region are displaced with respect to one another in relative fashion along the optical axis of the objective lens to different relative displacement positions. Intensity values of the laser beam are captured for a respective relative displacement position. A respective highest intensity value for a respective displacement position, a curve of the highest intensity values, and a reference relative displacement position from at least one maximum of the curve, are determined. Image(s) of the sample region is/are captured at the reference relative displacement position.

Method and microscopy system for recording an image

A method is used for acquiring and displaying an immunofluorescence image of a biological sample. During a first operating state, the sample is continuously illuminated using the excitation radiation. A relative position between the sample and an optical system, which guides the fluorescent radiation to an image sensor, is changed in dependence on a movement gesture of a user, and a corresponding digital image is displayed. Upon detection of a termination of the movement gesture, a change is made from the first operating state to a second operating state. In the second operating state, the sample is firstly illuminated using the excitation radiation to excite the fluorescent radiation of the fluorescent pigment. An acquisition of fluorescent radiation emitted by the sample then takes place and a corresponding, further digital image is generated. In the second operating state, the acquisition of the emitted fluorescent radiation and the illumination of the sample are terminated after lapse of the second acquisition duration.

Method and device for acquiring and displaying an immunofluorescence image of a biological sample

An apparatus for automated examination of biological material () includes a microscope () with a cross table () with the biological material () being arranged on the cross table () between an object slide () or an analysis plate and a cover (); a light source (); an evaluation unit; a device for automatic focusing (); an image recording unit () for recording an image of the biological material () enlarged via of an object lens () of the microscope () and for transferring the image to the evaluation unit; and a biochip (). Labeling () is provided on a surface of the biochip (). Focusing is executable by targeted movement of the cross table () based on detection of the labeling (), and a target interval is determinable by determination of a location and/or a position of the labeling, within which focusing of the biological material takes place.

Method and apparatus for automatic focusing of substrates in flourescence microscopy

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Luebeck, Germany

Markus Morrin