City of Hope

Markus Kalkum

Karine Bagramyan

Sanjeet Dadwal

Diana Diaz-Arevalo

James I Ito

Teresa B Hong

Paul J Yazaki

Miro Rusnak

Yoko Fujita-Yamaguchi

Sarah Shuck

Ismail H Al-Abdullah

Joseph M Lyons

Isaac Bishara

Daniel Roeth

Provided are methods, devices, and systems for a loop-mediated isothermal amplification (LAMP) reactions for detecting the presence of genetic material. A LAMP device includes an enclosure having outer walls configured to define an internal space. The LAMP device also includes a tray assembly configured to support a reaction vessel configured for holding a sample for amplification. The LAMP device also includes a plurality of light emitters configured to illuminate the reaction vessel for exciting a fluorescence of the sample in the reaction vessel. The LAMP device also includes an imaging device configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample. The LAMP device also includes a controller configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.

Loop-mediated isothermal amplification devices and systems for detecting genetic material

Disclosed herein are a recombinantS27S5 hemagglutinin (SHA), and homologues thereof, and a fusion protein of a fluorescent protein (such as GFP and mCherry1) and SHA or a homologue thereof, which specifically bind to carbohydrates, including oligomeric sugars that terminate in L-rhamnose or D-galactose. The SHA, SHA homologues, and fusion proteins can be used to detect a variety of microorganisms or cancer or tumor antigens.

Recombinant lectin and uses thereof

Provided herein are methods for detecting anprotease in a sample, diagnosing a subject with aspergillosis caused by aninfection based on the presence of anprotease in a sample, and methods of aspergillosis treatment that incorporate these diagnostic methods. In certain embodiments, theprotease is Asp f2, and theinfection is caused, or. Also provided herein are antibodies and kits for use in these methods, including novel antibodies specific for Asp f2.

Methods, compositions, and kits for detection of aspergillosis

Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

Methods for detection of botulinum neurotoxin

Substrates for detection of botulinum neurotoxin

Provided herein are novel fluorogenic and bioluminescent bacterial protease substrates that contain at least one bacterial protease cleavage site. In some embodiments, the proteases that cleave the protease substrates are bacterial proteases including those used for tissue dissociation, cell isolation, and cell detachment, such as the preparation of islet cells. Additionally, the novel protease substrates described herein may be used to assess the activity of certain bacterial proteases involved in isolating primary cells and stem cells. Methods and protease detection kits using the novel protease substrates are also provided herein.

Methods, compositions, and kits for detection of bacterial proteases

Provided herein, inter alia, are single chain antibody binding domains (VHHs) for use in conjunction with the Assay with a Large Immuno-sorbent Surface Area (ALISSA). Engineered and affinity matured VHHs are used as affinity reagents in the ALISSA resulting in an exceptionally sensitive and precise method for the detection of toxins at miniscule concentrations. Thus, provided herein are methods as well as reagents that can be used to detect the presence of botulinum neurotoxins in quantities well below 1 pg/mL which corresponds to a lethal concentration under presumed equal distribution throughout the human body.

Single chain antibody domains for detection of anti-botulinum neurotoxin domains and methods of their use

The present invention relates to compositions and methods in preventing and/or treating diseases caused by. In particular, the present invention is directed tovaccine preparations and methods of making and using thereof.

[object Object]

Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin,lethal factor, human chitinases, andproteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

Growing community of inventors

Azusa, CA, United States of America

Markus Kalkum