Eth Zurich

Eidgenossische Technische Hochschule Zurich

Limmatech Biologics Ag

University of Zürich

Markus Aebi

Umesh Ahuja

Michael T Kowarik

Saba Amber

Karin Ilg

Michael L Wetter

Manuela Mally

Michael Wacker

Amirreza Faridmoayer

Michael Hengartner

Kaspar Locher

Flavio Schwarz

Flavio Schwartz

Christian Lizak

Noemi Kalin

Niels Van Der Velden

Michael Freeman

Jorn Piel

Markus Kunzler

Timothy Keys

Ivan Hang

Chia-wei Lin

Christian A Lizak

Alex Butschi

Martin WÄLTI

Described herein are methods of producing glycosylated proteins in vitro and in vivo. The methods include using host cells to produce glycosylated proteins. Also described herein are glycosylated proteins produced using such methods and uses thereof.

Methods of producing glycosylated proteins

The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster ofand the homologous gene clusters fromandas well as related aspects.

Method for producing an n-methylated (poly) peptide

The present invention is directed to methods for identifying or designing (a) a potential oligosaccharide donor, (b) a potential oligosaccharyltransferase (OST), (c) a potential consensus sequence motif polypeptide, and/or (d) a potential glycosylation inhibitor for use in the oligosaccharyltransferase (OST)&#x2014;catalysed asparagine-linked ('N-linked') glycosylation, comprising the steps of generating a three-dimensional model of the catalytic domain and/or the polypeptide binding site of the oligosaccharyltransferase (OST) of, and designing or selecting a potential component selected from (a) to (d) which optimizes the stereo chemical complementarity of said three-dimensional model(s) and the potential component.

Rational design of components of the oligo-saccharyltransferase-catalysed asparagine-linked glycosylation

The present invention relates tocomprising at least pgl operon ofor a functional derivative thereof and presenting at least one N-glycan ofor N-glycan derivative thereof on its cell surface and, in particular, to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventingand optionallyinfections and methods for producing thesestrains.

[object Object]

The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.

Recombinant N-glycosylated proteins from procaryotic cells

The present invention relates tocomprising at least one pgl operon ofor a functional derivative thereof and presenting at least one N-glycan ofor N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventingand optionallyinfections and methods for producing thesestrains.

A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g.) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.

System and method for the production of recombinant glycosylated proteins in a prokaryotic host

Growing community of inventors

Wettingen, Switzerland

Markus Aebi