Glykos Finland Oy

Danisco US Inc.

Genencor International, Inc.

Teknologian Tutkimuskeskus Vtt Oy

Valtion Teknillinen Tutkimuskeskus

Teknologian Tutkimuskeskus Vtt

Vtt Technical Research Centre of Finland Ltd

Novartis International Pharmaceutical, Ltd.

Genecor International, Inc.

Roal Oy

Markku Saloheimo

Ann Westerholm-Parvinen

Anne Huuskonen

Anne Kanerva

Christopher Landowski

Merja Elisa Penttila

Jari Natunen

Juhani Saarinen

Jukka Hiltunen

Anna-Liisa Hänninen

Noora Salovuori

Tiina Pakula

Mari Hakkinen

Michael C Ward

Merja Penttilä

Marika Vitikainen

Jaakko Pere

Johanna Buchert

Kristiina Kruus

Barbara A Swanson

Mari J Valkonen

Christian Ostermeier

Benjamin Patrick Sommer

Heli Viskari

Ramon Wahl

Huaming Wang

Liisa Viikari

Matti Siika-Aho

Raija Lantto

Annamari Heiskanen

Bernhard Helk

Tiina Nakari-Setälä

Laura-Leena Kiiskinen

Karin Autio

Maija Tenkanen

Hanna Salo

Marjaana Rättö

Merja Penttil&auml;

Hanna Kontkanen

Nina Aro

Ville Pihlajaniemi

Jussi Joensuu

Emilia Selinheimo

Anne Leppänen

Heidi Salminen

Georg Schmidt

Eero Mustalahti

Yun Luo

Felipe Oseas Bendezu

Antti Nyyssola

Merja Penttila

Marjaana Ratto

Anna-Liisa Hanninen

Mari Haekkinen

Merja Penttilae

Merja PenttilÄ

Mari HÄKKINEN

The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.

Protease deficient filamentous fungal cells and methods of use thereof

Multiple proteases deficient filamentous fungal cells and methods of use thereof

Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.

Protein production in filamentous fungal cells in the absence of inducing substrates

The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such ascells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting oforcell.

O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

Multiple protease deficient filamentous fungal cells and methods of use thereof

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.

Polypeptides and active fragments of polypeptides having at least one esterase activity

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

Method for improved protein production in filamentous fungi

Described herein are compositions including filamentous fungal cells, such asfungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example,fungal cells, as the expression system.

Production of fucosylated glycoproteins

Growing community of inventors

Helsinki, Finland

Markku Saloheimo