Ge Healthcare Bio-Sciences Ab

Markku Hamalainen

Hakan Erik Roos

Peter Borg

Asa Frostell-Karlsson

A method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle comprises contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Concentration assay

A method of screening a plurality of fluid samples for the presence of species capable of specifically binding to a binding partner immobilized on a sensing surface of a sensor is disclosed. The method comprises contacting each sample with the sensing surface and a reference surface, and subjecting the sensing surface responses obtained for all samples to a computational process which comprises fitting al responses to a model equation for the relationship between a response at the sensing surface and the corresponding response at the reference surface. In an iterative process residuals above a pre-determined threshold value are removed, the model equation is adjusted, and all remaining samples are refitted to the adjusted model equation until the model equation at least substantially converges. Residuals above the predetermined threshold value are considered as species specifically binding to the binding partner. The method may be computer-implemented, and a computer program product therefore comprises instructions for causing a computer to perform the computational process.

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Uppsala, Sweden

Markku Hamalainen