Wyeth

Wyeth Holdings Corporation

Mark William Cutler

Earl Joseph Pursell

Kristen Elissa Syvertsen

Firelli Alonso-Caplen

Yun Kang

Mirelli Fino

Amadou Affrey Ouattara

Robert Repetto

Mireli Fino

Robert Anthony Repetto

A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 &#x3bc;m filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 &#x3bc;m filter and recovering the VSV in the filtered solution.

Purification processes for isolating purified vesicular stomatitis virus from cell culture

A process for producing isolated and purified respiratory syncytial virus (RSV) fusion (F) and attachment (G) glycoproteins in eukaryotic cell cultures infected with RSV cold-passaged, temperature-sensitive mutant subgroup A2 strain cpts-248/404 results in at least a 5-fold increase in F and G protein yields when compared with the parent A2 strain. Immunogenic compositions comprising the F and/or G protein(s) produced by this process can be formulated for in vivo administration to a host to confer protection against disease caused by RSV.

Process for increasing RSV surface glycoprotein yields using a mutant strain of RSV

Growing community of inventors

Bloomingdale, NJ, United States of America

Mark William Cutler