University of Alabama

Resolved Technologies, Inc.

Shot Hardware Optimization Technology, Inc.

Mark Wells

John C Vellinger

Mark S Deuser

Kenneth W Barton

Joseph D Ng

John W Symonds

Greg Jenkins

Juan-Manuel Garcia-Ruiz

Jose A Gavira-Gallardo

A sample device and method for analyzing a sample are claimed. The sample device is a flat disc containing channels and wells for directing a sample to reagents located in the disc and for mixing the sample with the reagents. The disc is mounted on an analyzer and the sample is pumped into the disc, divided into a plurality of sub-sample, and at least some of the sub-samples are mixed with a number of reagents. The resultant analytes are analyzed spectrophotometrically for a determination of the concentration of various substances in the sample. The present invention permits multiple tests to be performed quickly and automatically with minimal operator involvement.

Device for multiple tests from a single sample

A bioreactor apparatus and cell culturing system is provided for the automated cultivation and processing of living cells remotely both on earth and in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its cylindrical axis while allowing the entrance of fresh or recycled fluid and the removal, optionally, of spent medium, medium to be recycled or filtered or unfiltered medium for the collection of samples. A method of exchanging gases between the culture medium and ambient gases is provided. A fresh-medium storage bag and peristaltic pump is used for batch feeding, perfusion or sample collection. An enclosure and manifold representing an additional level of chemical containment and a series of pinch valves for the periodic collection of samples of suspended cells or cell-free medium is disposed therein together with a humidity control system. The bioreactor is computer controlled in order to control all functions including rotation of the reactor vessel, feeding fresh medium, perfusing the reactor vessel, timed collection of samples of fluid from the reactor, selecting between collecting cells or cell-free supernatant. A sealed compartment for sample-collection bags provides a level of chemical containment for safety. A sealed external housing is used for all components of the device except the power supply and computer. An external loop and electronic video microscope provides real-time and recorded and/or transmitted observation of cells in the suspension.

Bioreactor apparatus and cell culturing system

The invention is a crystallization cassette and associated method for growing and analyzing macromolecular crystals in situ by X-ray crystallography. The cassette allows proteins (as well as other macromolecules) to be crystallized by the counter-diffusion method in a restricted geometry. Using this procedure, crystals can be adequately prepared for direct X-ray data analysis such that the protein's three-dimesional structure can be solved without crystal manipulation.

Crystallization cassette for the growth and analysis of macromolecular crystals and an associated method

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Athens, AL, United States of America

Mark Wells