Promega Corporation

Mark W Knuth

Mary Haak-Frendscho

John William Shultz

Susanne Selman

Scott A Lesley

Catherine E Villars

James Robert Hartnett

Kimberly K Knoche

A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.

High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications

A process for pro and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.

High level expression and facile purification of proteins, peptides and

Novel purified agarase enzymes from Flavobacterium sp. strain NR19 and cloned genes encoding the agarase enzymes are disclosed. Transformed host cells which express the novel agarase enzymes in isolatable quantities are also described. Also disclosed are antibodies specifically reactive with the novel agarases.

Isolated agarase enzyme from flavobacterium SP. strain NR19, cloned

Isolated agarase enzyme from flavobacterium sp. strain NR19, cloned

A process for isolating nucleic acids from agarose, and particularly regular agarose, is described wherein the agarose is augmented with a chaotropic substance and hydrolyzed by a novel purified agarase enzyme from Flavobacterium sp. strain NR19.

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Waunakee, WI, United States of America

Mark W Knuth