Purdue Research Foundation

Mark Thomas Morgan

Bruce Michael Applegate

Aparna Kothapalli

Viacheslav I Adamchuck

Lynda Louise Perry

Daniel R Ess

Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of0157:H7 to be propagated in nonpathogenic, thereby eliminating the safety and security risks of propagation in0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.

Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface

An apparatus for automatically measuring soil pH at a relatively large number of places in a field, and automatically creating a soil pH map by simultaneously measuring the position of the apparatus and storing the pH data in association with the location from which the corresponding soil sample was taken. The apparatus includes a wheeled chassis, a shank for exposing soil at a controlled depth, a sampling tray for collecting soil, a probe for measuring the pH of the soil, a water supply for cleaning the probe between measurements, a devise for measuring the location of the apparatus, and a computer for controlling the measurement cycle and recording the data.

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West Lafayette, IN, United States of America

Mark Thomas Morgan