University of Pittsburgh

Mark Shlomchik

Eduardo Schittler Neves

Adriana Turqueti Neves

Constantinos George Panousis

Alexander McIntyre Rowe

This document provides methods and materials involved in cloning functional TCRs from single T cells. For example, methods and materials for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, kits for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, methods for making such kits, collections of nucleic acid primers designed to amplify the entire coding sequence of both variable regions for each expressed V segment for functional &#x3b1;&#x3b2; or &#x3b3;&#x3b4; TCRs of a particular mammalian species, methods for using such collections of nucleic acid primers to clone functional TCRs from single T cells, and kits containing such collections of nucleic acid primers to clone functional TCRs from single T cells are provided.

Growing community of inventors

Pittsburgh, PA, United States of America

Mark Shlomchik