Amersham Pharmacia Biotech UK Limited

Ge Healthcare UK Limited

Nycomed Amersham Plc

Mark Samuel Briggs

Ian Edward Bruce

Adrian Christopher Simmonds

Alan L Hamilton

Albert Francis Santos

Elizabeth Swann

Christopher John Moody

James N Miller

John Griffiths

William Jonathan Cummins

John Anthony Smith

John Mama

Valerie Millar

Richard Martin West

The invention provides a method for measuring binding of a test compound to a G-Protein Coupled Receptor (GPCR). The invention also provides a method for identifying and measuring the effect that an agent has upon modulating the binding of a test compound to a G-Protein Coupled Receptor.

Method for measuring binding of a test compound to a G-protein coupled receptor

The present invention provides fluorescent cyanine dye-based nucleotide analogues, in which the cyanine dye is coupled to the &#x3b3;-phosphate group of a nucleoside triphosphate. Preferred embodiments of the invention are directed to fluorescent cyanine dye-based GTP analogues which may be employed in an homogeneous FRET-based assay to measure the binding of guanine nucleotides to GPCR polypeptides, or alternatively, to measure the effect of an exogenous ligand on GPCR protein binding.

Fluorescent nucleotide analogues

Dye intermediate and method

Benzophenoxazine dyes

Squarate dyes and their use in fluorescent sequencing method

Growing community of inventors

Cardiff, United Kingdom

Mark Samuel Briggs