Promega Corporation

Nycomed Amersham Plc

Other

Ge Healthcare Bio-sciences Corp.

Amersham Biosciences Corp

Amersham Pharmacia Biotech UK Limited

Mark McDougall

Dieter Klaubert

Chad Zimprich

Keith V Wood

Georgyi V Los

Lance P Encell

Monika G Wood

Aldis Darzins

Robert F Bulleit

Shiv R Kumar

Stephen J Dwight

Thomas A Kirkland

Satyam Nampalli

David Loakes

Tonny Johnson

Clifford Leslie Smith

Adrian Christopher Simmonds

Alan L Hamilton

Daniel M Brown

Fergal Hill

Carl Williams Fuller

Cesear Corona

John Richard Nelson

Poncho Meisenheimer

Anup Sood

William Jonathan Cummins

Paul Mitsis

Min Zhou

John J Macklin

Mei Cong

David Williams

Dan Brown

Constantin Neagu

Patrick Finn

Chad Zimbrich

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Covalent tethering of functional groups to proteins

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule

Provided herein are chelator constructs (e.g., nucleic acid, peptide, peptide nucleic acid, etc.) that sequester metal ions (e.g., Mg) under a first set of conditions and fail to sequester or release sequestered metal ions under a second set of conditions. In particular, nucleic acid constructs are provided that sequester metal ions (e.g., Mg) under conditions that favor secondary and tertiary structure formation and release or fail to sequester metal ions under conditions that disfavor the formation of such structures.

Reversible metal ion chelators

The present invention provides novel fluorescent dyes and kits containing the same, which are useful for labeling a wide variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis and/or treatment of disease conditions.

Carboxy X rhodamine analogs

Covalent tethering of functional groups to proteins and substrates therefor

Provided herein are fluorescent sensor agents, and methods of use and manufacture thereof. In particular, sensor agents are provided that exhibit a detectable change in fluorescence (e.g., fluorescence intensity) upon alteration of the pH of the surrounding environment (e.g., upon moving from one pH environment to another).

pH sensors

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as &#x2022; methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

The invention provides novel compounds and compositions of Formulas I and II, as well as methods of using them. The compounds can be used, for example, to quantify an amount of double stranded DNA in a sample subjected to nucleic acid amplification, or for real time monitoring of a nucleic acid amplification reaction. The compounds can be provided in a kit, for example, with other reagents and instructions for using the compounds and reagents.

Nucleic acid binding dyes and uses therefor

Compositions comprising a dehalogenase substrate and a contrast agent and methods of use

Compositions comprising a dehalogenase substrate and a radionuclide and methods of use

Growing community of inventors

Arroyo Grande, CA, United States of America

Mark McDougall