Dionex Corporation

Thermo Hypersil-keystone LLC

Mark Lee Tracy

Christopher A Pohl

Xiaodong Liu

Shanhua Lin Liehr

Xiadong Liu

Jung Un Baek

Jennifer M Nguyen

Ronald A Sherant

A plurality of chromatograms is run using a salt gradient with a range of constant pH values or using a pH gradient with a range of constant salt concentrations. A chromatography optimization algorithm can be used to identify at least one salt gradient chromatogram or at least one pH gradient chromatogram to establish optimized buffer conditions. The chromatography optimization algorithm can include a total number of peaks algorithm, a peak-to-valley algorithm, and/or a peak capacity algorithm.

Methods and systems for optimizing buffer conditions with liquid chromatography

A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure.

Method of packing chromatographic columns, packed chromatographic columns for use at high pressures and uses thereof

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Sunnyvale, CA, United States of America

Mark Lee Tracy