Massachusetts Institute of Technology

Mark Kyle Mimee

Timothy Kuan-Ta Lu

Christopher A Voigt

Robert James Citorik

Alex C Tucker

Provided herein, in some aspects, are tools (e.g., methods, compositions and nucleic acids) for building genetic circuits inandbacteria, as well as the bacteria containing the genetic circuits.

Gene expression in Bacteroides

Various aspects and embodiments of the invention are directed to methods and compositions for reversing antibiotic resistance or virulence in and/or destroying pathogenic microbial cells such as, for example, pathogenic bacterial cells. The methods include exposing microbial cells to a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease targeted to one or multiple genes of interest.

Growing community of inventors

Cambridge, MA, United States of America

Mark Kyle Mimee