Monsanto Technology LLC

Ecogen, Inc.

Monsanto Company

Mark J Rupar

William P Donovan

Annette C Slaney

Yuping Tan

James Arthur Baum

Chih-Rei Chu

Thomas M Malvar

Elizabeth Pease

Amy Jelen Gilmer

Timothy B Johnson

Willam P Donovan

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &#x3b4;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants

Disclosed is a novel &#x3b4;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle (). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

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Disclosed arestrains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novelgenes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Polynucleotide encoding coleopteran-toxic polypeptide

Coleopteran-toxic polypeptide compositions

Disclosed are transgenic plants, seeds, and progeny from such plants comprising a cryET29 DNA segment. The cryET29 DNA segment encodes CryET29 crystal protein which comprises SEQ ID NO:2 and exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle ().

Plants transformed with CryET29-encoding nucleic acids

Disclosed are nucleic acids encoding novel insecticidal &#x3b4;-endotoxin polypeptides, and vectors, transformed host cells, and transgenic plants that contain the nucleic acids. Also disclosed are methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Nucleic acids encoding coleopteran-toxic polypeptides and insect-resistant transgenic plants comprising them

Disclosed is a novel &dgr;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea ( ), as well as against coleopteran insects, including the southern corn rootworm ( ), western corn rootworm ( ), Colorado potato beetle ( ), Japanese beetle ( ), and red flour beetle ( ). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP

Disclosed are strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Lepidopteran-active Bacillus thuringiensis &dgr;-endotoxin compositions and methods of use

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &dgr;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP

Disclosed is a novel .delta.-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects

A purified and isolated cryIII-type gene was obtained from a novel B.t. strain. The gene has a nucleotide base sequence coding for the amino acid sequence illustrated in FIG. 1 . The 74.4 kDa protein produced by this gene is an irregularly shaped crystal that is toxic to coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica.

Growing community of inventors

Wilmington, DE, United States of America

Mark J Rupar