Butamax Advanced Biofuels LLC

E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Butamax (tm) Advanced Biofuels LLC

Other

Inbiose N.v.

Mark J Nelson

Yougen Li

Daniel P Okeefe

Der-Ing Liao

Mark Scott Payne

Robert Donald Fallon

Lori Ann Maggio-Hall

Larry Cameron Anthony

Hongxian He

Arthur Leo Kruckeberg

Jean-Francois Tomb

Steven Cary Rothman

Ranjan Patnaik

Lixuan Lisa Huang

Jessica McElvain

Albert Laurence Lu

Michael W Lassner

James Kevin Presnail

Rafael Herrmann

Michael G Bramucci

Gopal K Chotani

Caroline M Peres

Daniel P O'Keefe

Janet Ann Rice

Michael Dauner

Jahnavi Chandra Prasad

Ritu Bhalla

Andre R Abad

Hua Dong

Xiaomei Shi

Takashi Yamamoto

Clement W K Waine

Lisa L Huang

Susan B Lo

Ronnie D Erickson

Lisa A Laffend

Jessica Mcelvain

Katharine J Gibson

Daniel P O'keefe

A fermentation broth which includes a microbial cell which has been subjected to a condition under which the activity an endogenous transporter for 2' fucosyllactose is increased. Also provided are methods for increasing export of 2′ fucosyllactose from a microbial cell, methods for identifying an endogenous yeast transporter of 2′ fucosyllactose, and microbial cells genetically engineered to increase the activity of an endogenous transporter of 2′ fucosyllactose.

Increasing activity of 2'fucosyllactose transporters endogenous to microbial cells

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased Kfor NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

Glycerol 3-phosphate dehydrogenase for butanol production

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

Host cells and methods for production of isobutanol

Glycerol 3- phosphate dehydrogenase for butanol production

Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.

Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes

Crabtree positive yeast cells that have endogenous expressed pyruvate decarboxylase genes inactivated and an engineered biosynthetic pathway utilizing pyruvate were found to have improved growth and product yield when glucose repression was reduced. These cells were able to grow in media containing a high glucose concentration.

Yeast production host cells

Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated fromthat have undergone co-factor switching to utilize NADH are described.

Ketol-acid reductoisomerase using NADH

Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated fromthat have undergone co-factor switching to utilize NADH are described.

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated fromthat have undergone co-factor switching to bind NADH are described.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Lepidopteran pest, decreases the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest, thereby limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides as set forth, or active variants and fragments thereof, wherein a decrease in expression of one or more sequences in the target pest has insecticidal activity. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In a specific embodiment, the pest is. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Compositions and methods for the suppression of target polynucleotides from lepidoptera

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. Target polynucleotides for specific protein classes and target polynucleotides set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof are provided, wherein a decrease in expression of one or more sequences in the target pest controls the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. Plants, plant part, bacteria and host cells comprising the silencing elements or an active variant or fragment thereof are also provided.

Compositions and methods for the suppression of target polynucleotides from the family aphididae

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains ofencoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

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Newark, DE, United States of America

Mark J Nelson