Other

Phynexus, Inc.

Mark J Abel

Douglas T Gjerde

Allen Burge

Ronald Jones

Lee Hoang

Chris Suh

Steven R Harrison

Thomas E Merrow

Robert A Stults

Jane J Laursen

George O Goodman

Jay Trow

An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation. In some embodiments, cells are purified from biological samples.

Method and apparatus for pipette tip columns

An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.

Method and device for sample preparation

The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.

Switch connections in a communication network are represented and displayed to a user, enabling the user to provide switch request signals based on the state of the connections. The representation is presented on the display of a workstation, and the user provides a sequence of switch request signals with the workstation's keyboard and mouse. A control procedure executed by the workstation generates a sequence of switch control signals based on the switch request signals. The switch control signals are provided to a switching server that converts them into commands to a switch that, in turn, switches connections between communication devices, such as cameras, monitors and microphones. These devices are positioned in groups, each group with one of the workstations. The representation may include a separate display feature representing each group, each group's display feature being at a separate location. Connections may be represented by lines between the groups that are connected. The control procedure also provides data so that the representation shows the current connections, including update data when connections are switched. This update data is based on a connection data structure in a shared file accessible by all the workstations on a network. A locking file in the same shared file system prevents interference between switch request signals from different users. A user can modify the switch control signal sequence based on a switch request signal sequence by accessing a signal data structure used by the control procedure to generate the switch control signals.

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Littleton, CO, United States of America

Mark J Abel