Dxcover Limited

R.p. Scherer Corporation

Mark Hegarty

Matthew Baker

Holly Jean Butler

David Palmer

Howard Norman Stevens

Abdul Rashid

Massoud Bakhshaee

James Leonard Mackie

James Robertson Kelso

Matthew J Baker

A method of detection of a status of cells within a cell culture is described. The method is based on comparing an IR spectrum of a test sample obtained from the cell culture with an IR spectrum of a control sample or samples or with an IR spectrum of a sample or samples obtained at an earlier time point in the cell culture and correlating differences between the spectra with the status of the cells. The status may be classified into healthy or unhealthy. The test sample may or may not contain cells. The test sample may contain cell fragments, cell components or cell media, or may be cell supernatant. The comparison may be performed using a predictive model based on pattern recognition algorithms, such as support vector machines SVM, linear discriminant analysis LDA, principal component discriminant function analysis PC-DFA, neural networks, or random forests). The analysis results may be used to monitor and/or control the cell culturing process.

IR spectroscope cell culture analysis

A sample container () for use in a ATR-FTIR spectrometer, comprises an internal reflection element 'IRE' (), the IRE comprising a first surface () and a second surface (). The first surface () is configured to receive a sample () and the second surface () is an infrared beam-receiving surface. The IRE () forms at least a portion of a wall of the sample container (), such that in use, when a sample () is provided on the first surface () of the IRE (), the sample () is provided within the sample container ().

Sample container with integrated internal reflection element

A sample slide () for use in a spectrometer (), wherein the sample slide comprises a plurality of sample-receiving portions (-) provided on a sample side () of the slide, and a plurality of beam-receiving portions (-) provided on a beam-receiving side () of the slide, each beam-receiving portion being arranged opposite a respective sample-receiving portion, and wherein each beam-receiving portion is configured to act as an internal reflection element (IRE). A device () for use with a spectrometer () comprises a stage () configured to receive a sample slide (); and a moving mechanism () configured to move the sample slide relative to a sample-measuring location () of the device. Associated methods for preparing a sample and measuring a sample are also disclosed.

Infra-red spectroscopy system

Capsule construction

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East Kilbride, United Kingdom

Mark Hegarty