University of Washington

Visiongate, Inc.

Other

Nortis, Inc.

Gaafar, Ahmed

Selim, Riham M.

Fauver, Mark

Mark Fauver

Eric J Seibel

J Richard Rahn

Alan C Nelson

Per G Reinhall

Michael G Meyer

Thomas Neumann

Chris Brown

Quinn Y J Smithwick

Janet Crossman-Bosworth

Anna V Tourovskaia

Julia Oi Yan Yu

Roger H Johnson

Wei-Chih Wang

Christy A Lancaster

Riham M Selim

Saniel D Lim

Ahmed Gaafar

Wei-Chi Wang

Anna Tourovskaia

The present disclosure provides a fluidic device including a first inlet, an outlet, and a channel positioned between the first inlet and the outlet. The channel is in fluid communication with the first inlet and the outlet. The fluidic device further includes a second inlet positioned between the first inlet and the outlet. The second inlet is in fluid communication with the channel. The fluidic device further includes a pump in fluid communication with the second inlet. The pump is configured to provide a first volume of pulsatile flow to the channel. The first volume of pulsatile flow is greater than about 50 μL per pulse.

System, device, and method for biopsy removal from needles into a fluidic device

A monitoring system includes a light source, a light detector, a processor, and a non-transitory computer readable medium storing instructions that, when executed by the processor, cause the monitoring system to perform functions. The functions include illuminating, via the light source, a biological sample that is within a container while a fixation process is performed on the biological sample. The functions also include determining, via the light detector, that an optical transmittance of the biological sample satisfies a condition. The functions also include ceasing the fixation process in response to determining that the optical transmittance of the biological sample satisfies the condition.

Optical fixation monitor

In some embodiments, an apparatus, system, and method for activating a low-adhesion state of a thermal-sensitive tape is described. An example apparatus embodiment includes a light source and a temperature sensor. The light source is configured to illuminate a target area of the thermal-sensitive tape with a first spectrum of electromagnetic radiation to provide heating of the target area. The first spectrum including a first wavelength outside of a visible spectrum. The temperature sensor is configured to detect a second spectrum of electromagnetic radiation to approximate a temperature of the target area. The second spectrum includes a second wavelength different than the first wavelength.

Apparatus, system, and method for activating a low-adhesion state of thermal-sensitive tape

An example fluidic device includes an elastic tube, a first actuator coupled to an outer surface of the elastic tube between a first end and a second end of the elastic tube, and a second actuator coupled to the outer surface of the elastic tube between the first actuator and the second end of the elastic tube. The first actuator and the second actuator are configured to move apart from one another to transition a portion of the elastic tube positioned between the first actuator and the second actuator from a first condition to a second condition. A diameter of the elastic tube is greater in the first condition than in the second condition. The fluidic device also includes one or more rotatable components coupled to the first actuator and the second actuator which are configured to rotate the portion of the elastic tube positioned between the first actuator and the second actuator.

Biopsy sample manipulation and imaging system and methods for use

Three-dimensional printing pens and related systems and methods are provided. A three-dimensional printing pen may include a plurality of cartridges, each cartridge having a hollow cartridge body sized and shaped to include a colored thixotropic paste, a color of each thixotropic paste being different from a color of another thixotropic paste, and a plurality of nozzles, each nozzle fluidly communicatively coupled to a corresponding cartridge. The three-dimensional printing may further include a motor unit operably coupled to the plurality of cartridges, the motor unit operable to expel the colored thixotropic paste from each cartridge through the corresponding nozzle, a mixing tip fluidly communicatively coupled to the plurality of nozzles, the mixing tip sized and shaped to dispense therethrough one or more of the colored thixotropic pastes in a form of an output thixotropic paste, and a projection module. The projection module may include a light engine assembly that emits light having a wavelength, the light being projected to the output thixotropic paste to cure the output thixotropic paste that is dispensed from the mixing tip.

Full color 3D printing pen and related systems and methods

A method for 3D imaging of a biologic object () in an optical tomography system where a subcellular structure of a biological object () is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object () is moved relatively to a microscope objective () to present varying angles of view and the labeled biological object () is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object () and the microscope objective () within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object () from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ().

Functional imaging of cells with optical projection tomography

A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seededinto a channel in a matrix at to activate competencyof the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix. The sprouting parent vessels are grown until network forms

Method for creating perfusable microvessel systems

An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro-electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end of the cantilever.

Integrated optical scanning image acquisition and display

A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

3D imaging of live cells with ultraviolet radiation

An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

Focal plane tracking for optical microtomography

A method for reconstructing three-dimensional (3D) tomographic images. A set of pseudo-projection images of an object is acquired. Error corrections are applied to the set of pseudo-projection images to produce a set of corrected pseudo-projection images. The set of corrected pseudo-projection images are processed to produce (3D) tomographic images.

Method for image processing and reconstruction of images for optical tomography

A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.

System and method for processing specimens and images for optical tomography

A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

Method and apparatus for pseudo-projection formation for optical tomography

An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end the cantilever.

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Seattle, WA, United States of America

Mark Fauver