Applied Biosystems, LLC

Applera Corporation

Genapsys, Inc.

Life Technologies Corporation

Quantum Biosystems Inc.

Intel Corporation

The Perkin-elmer Corporation

Pe Corporation (ny)

Applera Corporation Applied Biosystems Group

Mark F Oldham

Eric S Nordman

Richard T Reel

Steven J Boege

Hesaam Esfandyarpour

Eugene F Young

Kevin S Bodner

Jon A Hoshizaki

Kosar Baghbani Parizi

Howard Greg King

Austin B Tomaney

Cheryl Heiner

Frank Lee

Susanne Baumhueter

Donald R Sandell

David Cox

John S Shigeura

Janice G Shigeura, Legal Representative

H Pin Kao

Linda G Lee

Sam L Woo

Aldrich N K Lau

Stephen E Moring

Willy K Wiyatno

Hongye Sun

Ian A Harding

Johannes P Sluis

Jason H Halsey

Alfred P Madden

David H Tracy

Dennis Lehto

Benjamin G Schroeder

Umberto Ulmanella

Stephen J Gunstream

Mark Borodkin

J Michael Phillips

Charles Stuart Vann

Mark T Reed

Kenneth J Livak

Adrian Fawcett

Jon M Sorenson

Gary Lim

Congcong Ma

Nigel P Beard

Achim Karger

Douglas Greiner

Barry J Blasenheim

Louis B Hoff

Todd A Weinfield

Douwe D Haga

Tracy L Ferea

Omead Ostadan

Greg Young

Shuji Ikeda

Xing Su

Tomoji Kawai

Joy Roy

Masateru Taniguchi

Charles R Connell

Yuh-Min Chiang

Janusz B Wojtowicz

David M Liu

Alexander A Dromaretsky

Liana Ilkova

Takahito Ohshiro

Ward Kevin Frye

Joon Mo Yang

Sergey V Ermakov

John R Van Camp

Maryam Shariati

Joseph Jackson

Min Yue

John O'Neill

Steven Menchen

Timothy M Woudenberg

John C Voyta

Reid Burton Kowallis

David Ruff

Dar Bahatt

James C Nurse

Michael S Albin

Richard Morris

Christina E Inman

John Bridgham

Michael R Gambini

Jerome A Mack

Theofilos Kotseroglou

Dennis E Mead

Peter Ma

Mark Shannon

George A Fry

Ben F Johnson

Marc Haberstroh

Jim Nurse

Joseph H Jackson

Howard Gregg King

Douglas W Grunewald

Jason E Babcoke

Eric Norman

Timothy Hunkapillar

There is disclosed a system for electrical charge detection comprising a nanoFET device. Also disclosed is a method of electrical charge detection for single molecule sequencing. The method includes attaching a macromolecule or assemblies thereof to a gate of a nanoFET device and flowing in a solution of charge tags, where a charge tag includes a nucleotide attached to a charge complex. The method also includes incorporating one charge tag into the macromolecule or assemblies thereof and cleaving the charge tags from the macromolecule or assemblies thereof. The method further includes detecting at least one of current and voltage from the nanoFET device.

Systems and methods for electronic detection with nanoFETs

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Apparatus, system, and method using immiscible-fluid-discrete-volumes

The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Systems and methods for genetic and biological analysis

A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

Scanning system and method for imaging and sequencing

An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.

Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength

A system and method for characterizing contributions to signal noise associated with charge-coupled devices adapted for use in biological analysis. Dark current contribution, readout offset contribution, photo response non-uniformity, and spurious charge contribution can be determined by the methods of the present teachings and used for signal correction by systems of the present teachings.

Signal noise reduction for imaging in biological analysis

Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Biomolecule sequencing devices, systems and methods

Devices and methods for detecting, identifying, and sequencing, compounds, complexes, and molecules are described. Electronic detection is combined with optical excitation to determine the presence or identity of an analyte of interest. Embodiments of the invention additionally provide devices and methods that allow highly parallel nucleic acid sequence determination.

Integrated photonic electronic sensor arrays for nucleic acid sequencing

A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Systems and methods for automated reusable parallel biological reactions

A system for detecting a biomolecule comprises a nano-gap electrode device including a first electrode and a second electrode adjacent to the first electrode. The first electrode can be separated from the second electrode by a nano-gap that is dimensioned to permit the biomolecule to flow through the nano-gap. The nano-gap can have at least a first gap region and a second gap region. The second gap region can be oriented at an angle that is greater than zero degrees with respect to a plane having the first gap region. The system can further include an electrical circuit coupled to the nano-gap electrode device. The electrical circuit can receive electrical signals from the first electrode and the second electrode upon the flow of the biomolecule through the nano-gap.

Nano-gap electrode pair and method of manufacturing same

Growing community of inventors

Los Gatos, CA, United States of America

Mark F Oldham