E.i. Dupont De Nemours and Company

Alliance for Sustainable Energy, LLC

Danisco US Inc.

Genencor International, Inc.

Midwest Research Institute National Renewable Energy Laboratory Div.

Mark Emptage

Perry G Caimi

Gregory Marshall Whited

Paul Veikko Viitanen

Sharon Loretta Haynie

Min Zhang

Yat-Chen Chou

Jeff P Pucci

Lisa Anne Laffend

Mary Ann Franden

Xu Li

William Dean Hitz

Colin Mitchinson

Benjamin S Bower

Edmund A Larenas

Suzanne E Lantz

Bradley R Kelemen

Thijs Kaper

Steven Sungin Kim

Carol M McCutchen

Keith Dumont Wing

Carol M Mc Cutchen

Megan Yee Hsi

Carol M Mccutchen

Jeff Pucci

Greg Whited

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.

Glycosyl hydrolase enzymes and uses thereof

Strains of xylose utilizingwith improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizingin media containing high sugars, acetic acid, ammonia, and ethanol.

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Production of ethanol using a strain of xylose-utilizingwith a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.

Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas

Xylose-utilizingstrains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.

Ethanol production in fermentation of mixed sugars containing xylose

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of antransformed with thedha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence inof a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

Process for the biological production of 1,3-propanediol with high titer

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of antransformed with thedha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild typeIn another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence inof a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an transformed with the dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

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Wilmington, DE, United States of America

Mark Emptage