Dsm IP Assets B.v.

Evonik Operations Gmbh

Mark Edward Nejako, Ii

Neil Francis Leininger

Christian Rabe

Michael B Johnson

Ginger Marie Shank

Martin Heining

Kirt Lyvell Matthews, Sr

Holger Pfeifer

Jochen Lebert

Xiao Daniel Dong

Vinod Tarwade

David Allen Tinsley

Shannon Elizabeth Ethier Resop

Michael Diehl

Annika Hartmann

The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.

Method of isolating lipids from a lipids containing biomass

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Woodbine, MD, United States of America

Mark Edward Nejako, Ii