Wake Forest University

Keraplast Technologies, Ltd.

Southwest Research Institute

Electric Power Research Institute Incorporated

Ralph H. Hill

Andrew J. Phillips

Joseph N. Mitchell

Dennis S. Rushforth

Mark E. Van Dyke

Henry W. Jr. Oviatt

Mary C. Marshall

Spring M. Haby

Mark E Van Dyke

Cheryl Renee Blanchard

Scott F Timmons

Robert Allen Smith

Arlene J Siller-Jackson

Anthony Atala

James J Yoo

George J Christ

Sang Jin Lee

Patrick W Whitlock

Thomas L Smith

Geoffrey Dearnaley

Mary C Marshall

Ralph Henry Hill, Jr

Joseph N Mitchell

Luke Burnett

Justin M Saul

Dennis S Rushforth

Andrew J Philips

Roche De Guzman

Spring M Haby

Thaleia Teli

Henry W Oviatt, Jr

Cristin M Ferguson

Michael Callahan

Andrew J Phillips

Patrick Whitlock

Ralph H Jr Hill

Henry W Jr Oviatt

The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

Directed stem cell recruitment

A malleable bone graft composition is described. The composition comprises: (a) keratose; (b) particulate filler; (c) antibiotic; and (d) water. The invention may be provided in sterile form in an container, and optionally lyophilized. Methods of treating a fracture with such compositions are also described.

Keratin bioceramic compositions

Methods are provided to produce optimal fractionations of charged keratins that have superior biomedical activity. Also provided are medical implants coated with these keratin preparations. Further provided are methods of treating blood coagulation in a patient in need thereof.

Coatings and biomedical implants formed from keratin biomaterials

Provided herein are keratin compositions (e.g., keratin gels, scaffolds, particulates, and the like) including a compound of interest, useful for release and/or delivery of the compound of interest (e.g., in vivo or in vitro). In some embodiments, the composition is a composition formulated for controlled release of the compound of interest.

Controlled delivery system

Fractionation of charged keratin

A keratin hydrogel matrix serves as an effective acellular scaffold for axonal regeneration and facilitates functional nerve recovery.

Nerve regeneration employing keratin biomaterials

Renal tissue regeneration

A bone graft composition is described, with one or more of: (a) keratose; (b) kerateine; (c) optionally, particulate filler; (d) optionally, an antibiotic; and (e) water or saline. The composition may be provided in sterile form in a container, and optionally lyophilized. Methods of treating a bone deficiency or fracture making use of such compositions are also described.

Keratin compositions for treatment of bone deficiency or injury

Methods are provided for producing a bioscaffold from natural tissues by oxidizing a decellularized tissue to produce a bioscaffold having pores therein. The pore size and porosity is increased to better accommodate intact cells so that live cells can better infiltrate and inhabit the bioscaffold. The bioscaffold may be freeze-dried or lyophilized, sterilized and (optionally) aseptically packaged for subsequent use. A further aspect of the present invention is a bioscaffold produced by the processes described herein. Methods of treatment using the bioscaffold as a graft or as a biomedical implant for implantation are also provided. Also provided are methods of seeding a bioscaffold with mammalian cells, wherein the seeding carried out either in vitro or in vivo, and wherein a bioscaffold produced as described herein is utilized for said seeding.

Structurally modified acellular tissue engineering scaffolds and methods of production

Provided are keratin compositions useful in cell culture. In some embodiments the keratins are biocompatible, promote cell growth, promote cell adhesion and provide an excellent substrate for cell culture. Keratin compositions described herein may be used as coatings, gels, three-dimensional scaffolds, additives to cell culture media, microcarriers, etc. The keratin substrates may also be used to deliver cells, e.g., for cell therapy applications.

Keratin biomaterials for cell culture and methods of use

A malleable bone graft composition is described. The composition comprises: (a) keratose; (b) particulate filler; (c) antibiotic; and (f) water. The invention may be provided in sterile form in an container, and optionally lyophilized. Methods of treating a fracture with such compositions are also described.

Provided herein are methods of producing a meniscus scaffold to remove material and increase the pore size and porosity therein. In some embodiments, methods include seeding the tissue with allogeneic or autogeneic cells. Bioscaffolds produced by the processes described herein are also provided, as are methods of treating a subject in need of a bioscaffold implant.

Tissue engineered meniscus scaffolds and methods of use

Growing community of inventors

Winston-Salem, NC, United States of America

Mark E Van Dyke