United States of America, As Represented by the Secretary of Agriculture

The United States of America As Represented by the Secretary of

Other

Kansas State University Research Foundation

Zoetis Services LLC

Southern Illinois University

Mark C Jenkins

Ronald Fayer

James Trout

Ron Fayer

Hyun S Lillehoj

Harry D Danforth

Michael D Ruff

John B Dame

Jitender P Dubey

Celia O'Brien

Nicola C Lally

Wenbin Tuo

Michael Tilley

Vjollca Konjufca

Yan Zhao

Jonathan L Schaeffer

Steven L Upton

Joseph T Persyn

Monica Santin

Raymond H Fetterer

Dumitru Macarisin

The drinking water-based avian coccidiosis vaccine formulation and delivery system is structured to deliver a vaccine containing liveoocysts to poultry house brood chamber chicks. The vaccine is delivered to the chicks in a diluted form through the poultry house drinking water system. The chicks are inoculated with the drinking water-based avian coccidiosis vaccine when they consume the water containing the vaccine. The timing of the chicks' drinking water access to the diluted drinking water vaccine is critical. During the inoculation process, water is temporarily withheld from the chicks for a waiting period of about 3-5 hours, and access to the diluted drinking water-based vaccine is limited to an accessibility period of about 2 hours. Additionally, metering valves and/or terminal metering valve assemblies are manually or automatically closed when the when the drinking water lines are fully charged with the diluted vaccine.

Poultry drinking water-based vaccine delivery system and method

Provided herein are immunogenic compositions containing recombinant proteins capable of presenting all, or antigenic portions of, the, andIMP1 protein in developing active immunity to, and control of, coccidiosis. Also provided are methodologies of using the immunogenic compositions for administration to poultry and other animals in the control of coccidiosis. Nanoparticle-conjugated rIMP1 immunogenic compositions and methods of making and using them are provided.

Recombinantprotein delivered as nanoparticles

Safe and effective gel-bead vaccines for treating domesticated birds for diseases caused by cyst-forming protozoa, especially for coccidiosis.

Gel vaccine delivery system for treating poultry

Immunolocalization of &#x3b2;- and &#x3b4;-giardin introphozoites revealed that both giardins are strictly associated with the ventral disc. Optical sectioning of immunolabeled ventral disc, together with quantitative co-localization of &#x3b4;- and &#x3b2;-giardin immunoreactivity, demonstrated that &#x3b4;-giardin is primarily localized to the ventral side, and &#x3b2;-giardin is localized to the dorsal side of the ventral disc. Antibodies to &#x3b4;-giardin and &#x3b2;-giardin can both be used as diagnostic agents; anti-&#x3b4;-giardin antibody can be used as a therapeutic reagent to inhibit binding of trophozoites to host cells.

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is the causal agent of bovine neosporosis which results in high levels of abortion. The protective efficacy of twoantigens:cyclophilin (NcCyP) and NcSRS2 was evaluated. Mice vaccinated with recombinant (r) NcCyP, rNcSRS2, and the combination rNcCyP plus rNcSRS2, formulated with adjuvant ImmuMax-SR&#xae; and CpG were challenge-infected 3 weeks following the booster immunization and necropsied 3 weeks after the challenge infection. Mice vaccinated with rNcCyP, rNcSRS2, or the combination of rNcCyP and rNcSRS2 responded with high levels of NcCyP- or NcSRS2- specific antibodies. Mice which received vaccines formulated with either rNcCyP or the combination rNcCyP and rNcSRS2 had a higher (p&#x3c;0.01) percent protection when compared to the mock- or non-vaccinated mice. Groups immunized with rNcSRS2 alone exhibited slightly lower levels of protection. Results indicate that NcCyP is a highly efficacious vaccine candidate useful in protection againstinfection.

Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The proteins are effective for the immunization of a variety of animals against , particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

41 kDa Cryptosporidium parvum oocyst wall protein

Antigens for the detection of antibodies to Neospora parasites for the diagnosis of neosporosis have been identified. Recombinant antigens may be produced by expression of DNA sequences derived from Both antigens are capable of detecting antibody responses in animals experimentally inoculated with but show no evidence of cross-reactivity with serum from animals inoculated with closely related parasites such as or Sarcocystis species.

Recombinant antigens useful for the serodiagnosis of neosporosis

Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The proteins are effective for the immunization of a variety of animals against,particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

Cloning and expression of a DNA sequence encoding a 41 kDa cryptosporidium parvum oocyst wall protein

Recombinant proteins have been developed for the immunization of animals against cryptosporidiosis. The Proteins are effective for the immunization of a variety of animals against Cryptosporidium parvum, particularly for the production of hyperimmune colostrum that may be used to confer passive immunity against the parasite. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

DNA sequence encoding surface protein of cryptosporidium parvum

Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences. The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis. The DNA sequences of the present invention comprise cloned genes or fragments thereof that code on expression for an antigenic protein that activates white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein.

Growing community of inventors

Davidsonville, MD, United States of America

Mark C Jenkins