Histogen, Inc.

Advanced Tissue Sciences, Inc.

Other

Mark Baumgartner

Gail K Naughton

Kyle Nickey

Frank Ziegler

Mark A Applegate

Francis C Zeigler

Dawn Orton Applegate

James H Flatt

John W Bennett

Fred Schramm

Lee Laiterman

Robert Hardin

John Danssaert

Nicole Bloom

William R Tolbert

Christine Kurjan

Frank Zeigler

The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions

Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions for methods of treating tissue injury

The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

Soluble composition for promoting hair growth produced by hypoxic culture of fibroblasts cells

Repair and/or regeneration of cells with a composition produced by culturing fibroblast cells under hypoxic conditions

Low oxygen tension and BFGF generates a multipotent stem cell from a fibroblast in vitro

Extracellular matrix compositions produced under hypoxic culture conditions

A tissue culture chamber is disclosed. The chamber is a casing that provides for growth of three-dimensional tissue, and more specifically for the growth of skin tissue, that can be grown, preserved in frozen form, and shipped to the end user in the same aseptic container. The tissue culture chamber includes a casing comprising a substrate within the casing designed to facilitate three-dimensional tissue growth on the surface of the substrate. The casing includes an inlet and an outlet port which assist the inflow and outflow of media. The casing also includes at least one flow distributor. In one embodiment, the flow distributor is a baffle, which is used to distribute the flow of the media within the chamber to create a continuous, uniform piece of three-dimensional tissue. In a second embodiment, the flow distributor is a combination of deflector plates, distribution channels, and a flow channel. In each embodiment, the casing further includes a seal so as to ensure an aseptic environment inside the chamber during tissue growth and storage.

Apparatus for the growth and packaging of three dimensional tissue

An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants. Due to the flexibility of the treatment chambers, rigid spacers are also provided which ensure even fluid distribution within the chambers during treatment.

Apparatus for the large scale growth and packaging of cell suspensions

Growing community of inventors

San Diego, CA, United States of America

Mark Baumgartner