Siemens Healthcare Diagnostics Gmbh

Grifols Diagnostic Solutions Inc.

Mark Baumeister

Thomas Battersby

Jody Berry

Jesse Brooks

Stacey Tam

Felix Kleshik

Jody Melton Witt

Sodany Son

The methods and reagents described herein can be used to shorten incubation times in hybridization assays. As demonstrated in the examples, we have identified specific sulfonic acid polymers and hybridization conditions that lead to significantly shorter incubation times (e.g., signals after three hours that are comparable to signals that could traditionally only be obtained after overnight incubation). In some embodiments, shorter incubation times are achieved by adding the sulfonic acid polymer(s) during the hybridization process. Alternatively or additionally, in some embodiments, shorter incubation times are achieved via changes to the hybridizing conditions, e.g., by reducing the hybridization volume, increasing the salt concentration, andor increasing the probe concentration (capture extender probe andor label extender probe).

Methods and reagents for shortening incubation times in hybridization assays

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Richmond, CA, United States of America

Mark Baumeister