Biofire Diagnostics, LLC

University of Utah Research Foundation

Biofire Defense, LLC

Biofire Defence, LLC

Biofire Defense, Llc. Et Al.

Biofire Diganostics, LLC

Biomerieux, Inc.

Idaho Technology, Inc.

Mark Aaron Poritz

Kirk M Ririe

Randy P Rasmussen

Gary Clark Kessler

Michael R Newswander

Stewart Benjamin Smith

David E Jones

Anne Jeannette Blaschke-Bonkowsky

Carl Thomas Wittwer

Luming Zhou

Robert John Crisp

Margarita Rogatcheva

Christopher Paul Pasko

Tyler Lane Healy

Kenneth K C Bramwell

Cynthia L Phillips

Dave E Jones

Matthew Kam Jones

Robert Cornelius Trauscht

Ann Michelle Demogines

Usha K Spaulding

Kirk Max Ririe

Come Henri Albert Barranger

Stephane Christian Jacques Magro

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.

Self-contained biological analysis

Systems, methods, and apparatus are provided for external control testing of an assay system.

Methods and systems for validation of a nucleic acid amplification assay

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.

High density self-contained biological analysis

Methods, sample vessels, and instruments are provided for quantitative and semi-quantitative amplification.

Methods for quantitative amplification

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immune-PCR.

Methods and containers are provided for identifying a species, illustratively a bacterial species. Illustrative methods comprise amplifying various genes in the nucleic acid from the bacterial species in a single reaction mixture using pairs of outer first-stage primers designed to hybridize to generally conserved regions of the respective genes to generate a plurality of first-stage amplicons, dividing the reaction mixture into a plurality of second-stage reactions, each using a unique pair of second-stage primers, each pair of second-stage primers specific for a target bacterial species or subset of bacterial species, detecting which of the second-stage reactions amplified, and identifying the bacterial species based on second-stage amplification. Methods for determining antibiotic resistance are also provided, such methods also using first-stage primers for amplifying genes known to affect antibiotic resistance a plurality of the second-stage reactions wherein each pair of second-stage primers specific for a specific gene for conferring antibiotic resistance.

Organism identification panel

Method and sample vessels are provided for amplification and sequencing of nucleic acids in a sample.

Sample to sequence

Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.

Rapid epidemiologic typing of bacteria

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyzes include high density nucleic acid amplification and detection and immune-PCR.

Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5' of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.

Primers for melting analysis

Growing community of inventors

Salt Lake City, UT, United States of America

Mark Aaron Poritz