Dyadic International (USA), Inc.

Other

Danisco US Inc.

Dyadic International, Inc.

Dyadic Nederland B.v.

Mark Aaron Emalfarb

Peter Jan Punt

Arkady Panteleimonovich Sinitsyn

Richard Paul Burlingame

Richard P Burlingame

Jan Cornelis Verdoes

Philip Terry Olson

Christine Marie Pynnonen

Alexander Vasilievich Gusakov

Cornelia Van Zeijl

Cornelius Van Den Hondel

Arie Ben-Bassat

Jean Christophe Bousson

Martine Parriche

Alexey I Antonov

Marco A Baez

Oleg N Okunev

Boris B Ustinov

Tatyana N Salanovich

Jacob B Visser

Jan Wery

Johannes Heinrich Visser

Cornelia Marie Johanna Van Zeijl

Vladimir Mikhaylovich Chernoglazov

Irina Vladimirovna Solovjeva

Cornelia Maria Johanna Van Zeijl

Phillip T Olson

Elena Vlasenko

Donald J Reum

Mark Baer

Paula Jouhten

Zhijie Jiang

Mark Gosink

Sandra Wihelmina Agnes Hinz

Oleg Nicolaevich Ocounev

Marja Hannele Ilmén

Sandra Castillo

Kari Tapio Koivuranta

Ronen Tchelet

Marie-Pierre Kazek

Jean Dubayle

Richard Kensinger

Teunis Cornelis Verwoerd

Mark R Alfenito

Isabelle Legastelois

Sy Emalfarb

Oleg Nicolaevich Okounev

Jacoba Van Der Meij

Marie-Clotilde Bernard

Marco Baez

Arkady P Sinitsyn

Alexander V Gusakov

Cornelia Maria Van Zeijl

The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species, capable of producing elevated amounts of nicotinamide riboside.

Nicotinamide riboside production in filamentous fungi

Recombinant expression of influenza virus surface proteins in the fungusstrain C1 is provided. The recombinant proteins are for use in influenza vaccine compositions.

Production of flu vaccine in

The present invention provides a new fungal production system comprising a fungal host strain ofwherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion ofstrain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous &#x3b2;-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion ofstrain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.

Fungal production system

The invention is generally directed to modified filamentous fungal host cells comprising one or more nucleic acids encoding one or more polypeptides under the control of one or more promoters that are functional in said cells. Methods of using the modified cells to express one or more polypeptides are also disclosed, including methods of screening cells transformed with one or more expression vectors comprising nucleic acids derived from synthetic or genomic nucleic acids including, cDNAs. Methods of purifying one or more polypeptides or complexes comprising one or more polypeptides expressed in the modified cells, intended for use as substrates in structure/function studies, as therapeutic agents, as diagnostic reagents, or as human or animal vaccines, are also disclosed.

Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi

This invention provides novel enzyme compositions using newly identified and isolatedenzymes, including CBH Ib CBH IIb, EG II, EG VI, &#x3b2;-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.

Construction of Highly efficient cellulase compositions for enzymatic hydrolysis of cellulose

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus, more particularly ofand mutants or derivatives thereof. It also covers transformants containingcoding sequences, as well expression-regulating sequences ofgenes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Transformation system in the field of filamentous fungal hosts

The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.

Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.

BX11 enzymes having xylosidase activity

The invention pertains to novel proteins corresponding toglycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to aglyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially astrain.

Expression-regulating sequences and expression products in the field of filamentous fungi

A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (). Work processes are modeled using an application framework () used to develop abstract, decision () process models. The decision () process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants () in the work process.

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The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glyclosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.

High-throughput screening of expressed DNA libraries in filamentous fungi

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Jupiter, FL, United States of America

Mark Aaron Emalfarb