Integrated Dna Technologies, Inc.

City of Hope

University of Iowa Research Foundation

Somagenics, Inc.

Other

Roche Finance Ltd

Foundation Medicine, Inc.

Walder, Joseph Alan

Behlke, Mark Aaron

Owczarzy, Richard

Lennox, Kimberly Ann

Jacobi, Ashley Mae

Mark Aaron Behlke

Joseph Alan Walder

John J Rossi

Dongho Kim

Michael Allen Collingwood

Richard Owczarzy

Scott Rose

Andrei Laikhter

Garrett Richard Rettig

Lingyan Huang

Yawfui Yong

Joseph R Dobosy

Christopher Anthony Vakulskas

Mikhail Podyminogin

James O McNamara

Frank J Hernandez

Alexander R Horswill

Jeffrey A Manthey

Nicole Mary Bode

Yong You

Kevin William Roberts

Eric Jeffrey Devor

Katie R Flenker

Ashley Mae Jacobi

Brian H Johnston

Anne Dallas

Heini Ilves

Qing Ge

Kristin Beltz

Mollie Sue Schubert

John Robert Havens

Karen Clark

Hyeon Kim

Beverly L Davidson

Mirna Jarosz

Caifu Chen

Doron Lipson

Paul B McCray, Jr

Liyang Zhang

Patrick Sinn

Zachary Zwirko

Hong Peng Jia

Frank Soo Juhn

Kim Lennox

Derek M Thomas

Michael R Marvin

Elliot Burghardt

Rolf Turk

Susan M Rupp

Anthony J Fischer

Maureen D Donovan

Shawn Allen

Sarah Franz Beaudoin

Adam Clore

Kerry Gunning

Katherine Perschbacher

Alexander R Howswill

Steven A Henck

Jessica Woodley

Bernice Thommandru

Beimeng Sun

Elliott Burghardt

Joesph Dobosy

Katie R Stockdale

John Froehlig, Jr

Kimberly Ann Lennox

Andrey Tataurov

Sarah Jacobi

Jessica Lister

Kerry B Gunning

Bernardo Moreira

Chris Vinci

Yawful Yong

Shale Anthony James

Joseph Dobosy

Scott D Rose

You Young

Scott Daniel Rose

James O Ii Mcnamara

Yon You

The present disclosure concerns polynucleotides and amino acids of. Cas12a (Cpf1) and methods for their use for genome editing in eukaryotic cells.

Methods using novel CPF1 mutations that enhance the DNA cleavage activity ofsp. CPF1

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays.

Oligonucleotide-based probes and methods for detection of microbes

This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

S. pyogenes CAS9 mutant genes and polypeptides encoded by same

This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

CAS9 mutant genes and polypeptides encoded by same

Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.

Modified double-stranded donor templates

The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from(P.a.),(T.kod), andorganisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases

Described herein are methods for the expression and purification of Cas13a and methods for detecting target RNA using Cas13a.

Expression and purification of Cas enzymes

Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.

Methods for production and quantification of unique molecular identifier-labeled beads

Methods for detecting on-target and predicted off-target genome editing events by providing a multiplex PCR reaction mixture with an on-target oligonucleotide primer and one or more off-target oligonucleotide primers and then hybridizing the on-target oligonucleotide primer and the one or more off-target oligonucleotide primers to target nucleic acid sequences, followed by cleaving blocking groups from the primers and extending the primers.

Method for detecting on-target and predicted off-target genome editing events

Cas9 mutant genes and polypeptides encoded by same

The invention is directed to modified oligonucleotide compositions and methods for selectively reducing unwanted nucleic acid contaminants and enriching for desired nucleic acid targets from complex genomic nucleic acid mixtures for sequencing applications. The modified oligonucleotide compositions include one or more modified groups that increase the Tof the resultant oligonucleotide composition.

T-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection

This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.

CRISPR-based compositions and methods of use

The present disclosure concerns polynucleotides and amino acids ofsp. Cas12a (Cpf1) and methods for their use for genome editing in eukaryotic cells.

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Growing community of inventors

Coralville, IA, United States of America

Mark Aaron Behlke