The United States of America, As Represented by the Secretary, Department of Health and Human Services

Mark A Pallansch

Larry J Anderson

Thomas G Ksiazek

Anthony Sanchez

Pierre E Rollin

William Allan Nix

Raymond Campagnoli

M Steven Oberste

David R Kilpatrick

Cara Carthel Burns

Paul A Rota

Dean D Erdman

Suxiang Tong

James A Comer

Cynthia Goldsmith

Stephan S Monroe

William J Bellini

Shannon L Emery

Charles D Humphrey

Joseph P Icenogle

Teresa C T Peret

Sherif R Zaki

Steven Oberste

Kaija Maher

Qi Chen

Byron T Cook

Jeanette Guarner

Richard F Meyer

Christopher D Paddock

Wun-Ju Shieh

Michael D Bowen

Disclosed herein is a newly isolated human coronavirus (SARS-CoV), the causative agent of severe acute respiratory syndrome (SARS). Also provided are the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of the SARS-CoV open reading frames, as well as methods of using these molecules to detect a SARS-CoV and detect infections therewith. Immune stimulatory compositions are also provided, along with methods of their use.

Coronavirus isolated from humans

The present invention discloses a method for detecting the presence of an enterovirus in a clinical sample. The invention additionally discloses a method for typing an enterovirus in a clinical sample. Both methods employ a set of primer oligonucleotides for reverse transcription and amplification that hybridize to conserved regions of the enterovirus genome, and that provide amplicons that include significant portions of the VP1 region that are characteristic of the various serotypes. In the typing method, the invention further provides a database consisting of nucleotide sequences from prototypical enteroviral serotypes, which is used to type the clinical sample by comparing the sequence of its amplicon with each prototypical sequence in the database. The invention additionally provides mixtures of primer oligonucleotides, and a kit for use in conducting the typing method that includes a mixture of the primer oligonucleotides.

Typing of human enteroviruses

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Lilburn, GA, United States of America

Mark A Pallansch