US Government As Represented by the Secretary of the Army

Other

The Walter Reed Army Institute of Research

Mark A Olson

Sina Bavari

Robert G Ulrich

Charles B Millard

Michael P Byrne

Robert W Wannemacher

Jaimee R Compton

Patricia M Legler

Ross D LeClaire

Robert G Ulrích

Disclosed herein ricin toxin A chain polypeptides having an engineered disulfide bond ((SS)RTA) and compositions thereof. The disclosed (SS)RTA polypeptides retain the immunological epitope of wild type RTA, lack detectable N-glycosidase activity or exhibit reduced N-glycosidase activity as compared to controls, and exhibit increased solubility, thermal stability and a lower tendency to self-aggregate as compared to RTA 198 and/or RTA 1-33/44-198. Also disclosed are immunogenic compositions that may be used to immunize a subject against ricin intoxication. Methods of immunizing against, treating, inhibiting, reducing and/and preventing ricin intoxication are disclosed.

Ricin vaccine and methods of making thereof

The present invention relates to genetically attenuated superantigen toxin vaccines altered such that superantigen attributes are absent, however the superantigen is effectively recognized and an appropriate immune response is produced. The attenuated superantigen toxins are shown to protect animals against challenge with wild type toxin. Methods of producing and using the altered superantigen toxins are described.

Bacterial superantigen vaccines

Disclosed herein are polypeptides and variants thereof comprising a polypeptide sequence having substantial identity to ricin A chain (RTA) that lack detectable N-glycosidase-rRNA activity or exhibit reduced N-glycosidase-rRNA activity as compared to controls and methods of making and using thereof. The polypeptides and variants have a greater solubility in aqueous solutions of physiological pH and ionic strength than RTA and also retain the integrity of the neutralizing immunological epitope of wild type RTA. Also disclosed are immunogenic compositions that may be used to immunize a subject against ricin intoxication. Methods of immunizing against, treating, and preventing ricin intoxication are disclosed.

Ricin toxin A-chain fragment for use as a vaccine

A snowboard has elongate runners integrally formed with the bottom surface of the snowboard to increase the downhill speed characteristics of the snowboard. The runners define a longitudinally extending channel thereinbetween. The cross-sectional thickness of the snowboard is thicker at the runners and becomes thinner along the channel to increase the stiffness of the snowboard without significantly increasing the weight of the snowboard.

Snowboard

Ricin vaccine and methods of making and using thereof

Growing community of inventors

Gaithersburg, MD, United States of America

Mark A Olson