Arizona State University

Arizona Board of Regents, a Body Corporate

Life Technologies Corporation

University of Utah Research Foundation

Other

National Institutes of Health, a Component of the US Dept. of Health & Human Services

The Governors of the University of Alberta

Mark A Hayes

Thomas J Taylor

Peter Williams

Ian R Gould

Daniel J B Williams

Paul Jones

Marc D Porter

Michael Sierks

LaKeta Kemp

Claire Crowther

Steven R Kjar

Neal W T Woodbury

Seth David Rose

Whitt F Woods

Vincent B Pizziconi

Antonio Garcia

Mikhail Skliar

Linda J Reha-Krantz

Jeffery C Hurd

Karl S Booksh

Anil K Vuppu

Samuel Hayes

Brennan Copp

Karl Booksh

Pierre Herckes

Stacy Kenyon

Michael Keebaugh

Linda B Bloom

Prasun Mahanti

Nolan A Polson

Sarah Staton

Alexis Ramirez

Noah Weiss

Robert Joseph Soto

The present disclosure provides systems and methods for separating one or more analytes within a fluid mixture, and characterizing and/or detecting properties associated with the one or more analytes. In some embodiments, the systems provided herein contain a dielectrophoresis device, such as a gradient insulator-based dielectrophoresis device (g-iDEP). The present disclosure provides systems and methods for separating and characterizing analytes using particle or nanoparticle tracking analysis (NTA). NTA offers various advantages because, particle size and concentration can be calculated in real time, allowing label-free and simultaneous characterization and separation of samples with mixed and unknown analytes.

Systems and methods for non-destructive isolation, concentration, and detection for unbiased characterization of nano- and bioparticles

This invention discloses a design a capillary column for use with electrochemically modulated liquid chromatography (EMLC). The capillary design, which results in a marked reduction in the flow of current through the column, enables the use of a two-electrode column construction that overcomes the mechanical and electrical shortfalls of the conventional standard bore design.

Two-electrode configuration for separations based on electrosorption in electrochemically modulated liquid chromatography (EMLC)

The present technology relates to improved device and methods of use of insulator-based dielectrophoresis. This device provides a multi-length scale element that provides enhanced resolution and separation. The device provides improved particle streamlines, trapping efficiency, and induces laterally similar environments. Also provided are methods of using the device.

Hyper efficient separations device

The present invention provides devices and methods to separate and concentrate target protein species at a microliter scale and to generate reagents to those variants with exquisite selectivity for specific protein isoforms using only picograms of target material.

Nanoscale process to generate reagents selective for individual protein variants

The present invention provides devices and methods to separate and concentrate target species. In some embodiments, a punctuated continuous microchannel or parallel processing (array-based) separations are provided, the microchannel having a plurality of sequential, constrictive insulating features to form a plurality of reservoirs including a first, second and third reservoir, and a plurality of constricted passageways including a first constricted passageway that connects the first reservoir to the second reservoir and a second constricted passageway that connects the second reservoir to the third reservoir. A voltage is applied to the microchannel to create different electrical fields and/or different dielectrophoresis (DEP) gradients at each of the plurality of constricted passageways in order to separate species that have differing ratios of electrokinetic mobility to dielectrophoretic mobility.

Punctuated microgradients for improved separations of molecules and particles

A mixing system includes a housing having a motor mount rotatably coupled thereto, the motor mount having a passage extending therethrough. A drive shaft is removably positioned within the passage of the motor mount. A cap includes a main body removably coupled to the motor mount and an actuator coupled to the main body so as to be pivotable between a first position and a second position with respect to the main body. The actuator producing a camming action when the actuator is pivoted such that when the actuator is in the first position, the actuator pushes the drive shaft against the motor mount so that the main body is locked to the motor mount and so that rotation of the motor mount causes rotation of the drive shaft and when the actuator is in the second position, the actuator is disengaged from the drive shaft and the cap is removable from the motor mount.

Drive shaft locking cap and related mixing system and method

The present invention provides for separation of bacterial species and serotypes using electrophoretic methods.

Bacterial identification

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

The invention relates to a method for identifying the source of shed bioparticles and an apparatus that implements the method. The method involves collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most effective in identifying their source, and gathering data from those bioparticles to form bioparticle signatures. The bioparticle signatures are then processed into a multi-dimensional vector which is then compared to the multi-dimensional vector derived from a standard using a pattern recognition strategy that identifies the source. The apparatus has a particle collection device to collect the sample, a transfer device that selects information-rich bioparticles and a detector that restricts the movement of the information-rich bioparticles. The restricted movement is then translated into a bioparticle signature.

System and method for automated bioparticle recognition

Growing community of inventors

Chandler, AZ, United States of America

Mark A Hayes