Advanced Tissue Sciences, Inc.

Skinmedica, Inc.

Massachusetts Institute of Technology

Allergan, Inc.

Allergen, Inc.

Mark A Applegate

Joan Zeltinger

Lee K Landeen

Gail K Naughton

Anthony Ratcliffe

Jonathan N Mansbridge

Andreas Kern

R Emmett Pinney

David L Horwitz

James H Flatt

Gregory N Stephanopoulos

Maria Flytzani-Stephanopoulos

Mark Baumgartner

Rahul Singhvi

Alvin Edward Peterson

John W Bennett

Seujeung Park

Dawn Orton Applegate

Stephen V Kemmerrer

Noushin Dunkelman

Scott Chesla

Jason Gee

William R Tolbert

Fred Schramm

Lee Laiterman

Robert Hardin

John Danssaert

Nicole Bloom

Christine Kurjan

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

Conditioned cell culture medium compositions and methods of use

Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.

An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing three-dimensional tissue constructs is disclosed. The apparatus includes a fluid reservoir, a pump, at least one treatment chamber, and a means for controlling media flow characteristics around a tissue construct disposed within the treatment chamber, and for controlling movement of the construct itself, so as to simulate a variety of physiologic conditions. One exemplary embodiment of the invention includes a means for applying an axial stress to the construct. Applying an axial stress to the construct during seeding and culturing results in a tissue-engineered construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of native vessels with normal physiologic function.

Apparatus and method for simulating in vivo conditions while seeding and

An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing cartilage constructs is disclosed. The apparatus generally includes a treatment chamber and a means for varying the fluid flow and pressure within the treatment chamber during seeding and culturing. The application of pressure to the cartilage construct improves both the uniformity of seeding throughout the construct and facilitates the flow of nutrients to and removal of waste products from cells embedded in the construct. In this manner, a tissue-engineered cartilage construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of cartilage with normal physiologic function may be formed.

Apparatus and method for sterilizing, seeding, culturing, storing,

A tissue culture chamber is disclosed. The chamber is a casing that provides for growth of three-dimensional tissue, and more specifically for the growth of skin tissue, that can be grown, preserved in frozen form, and shipped to the end user in the same aseptic container. The tissue culture chamber includes a casing comprising a substrate within the casing designed to facilitate three-dimensional tissue growth on the surface of the substrate. The casing includes an inlet and an outlet port which assist the inflow and outflow of media. The casing also includes at least one flow distributor. In one embodiment, the flow distributor is a baffle, which is used to distribute the flow of the media within the chamber to create a continuous, uniform piece of three-dimensional tissue. In a second embodiment, the flow distributor is a combination of deflector plates, distribution channels, and a flow channel. In each embodiment, the casing further includes a seal so as to ensure an aseptic environment inside the chamber during tissue growth and storage.

Apparatus for the growth and packaging of three dimensional tissue

An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants. Due to the flexibility of the treatment chambers, rigid spacers are also provided which ensure even fluid distribution within the chambers during treatment.

Apparatus for the large scale growth and packaging of cell suspensions

An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.

Cell-culturing apparatus and method employing a macroporous support

Growing community of inventors

San Diego, CA, United States of America

Mark A Applegate