Cargill Inc.

Lallemand Hungary Liquidity Management LLC

Sk Innovation Co., Ltd.

Valtion Teknillinen Tutkimuskeskus

Stellenbosch University

Danisco Sweeteners Oy

Rohm Enzyme Gmbh

Universiteit Stellenbosch

Marja Ilmen

Vineet Rajgarhia

Laura Ruohonen

Aristos Aristidou

Merja Elisa Penttila

Pirkko Suominen

Kari Koivuranta

Christopher K Miller

Stacey Olson

Merja Penttilä

Jaana Uusitalo

John E McBride

Allan C Froehlich

Matti Siika-Aho

Elena E Brevnova

Mark Mellon

Riaan Den Haan

Emily Stonehouse

Haowen Xu

Kristen M Deleault

Alan Benjamin Gilbert

John S Bardsley

Sanni Voutilainen

Anu Koivula

Deidre Willes

Sang Min Lee

Heikki O Heikkila

Marja T Paloheimo

Charles F Rice

Jae Yeon Park

Hans Söderlund

Anne K Warner

Marja-Leena Sarkki

Kristiina Takkinen

Heikki Ojamo

Chris Miller

Kari Kataja

Joong Min Park

Indraneel Shikhare

Outi Koivistoinen

Emile Van Zyl

Young Bin Seo

Kevin Roberg-Perez

Woo Chan Park

Maija-Leena Onnela

Merja Oja

Danie Lagrange

Merja Pentilla

Andrew Conley

Paula Jouhten

Elena Brevnova

Maija-Leena Vehkomaki

Naomi Thorngren

Jim Flatt

Merja Pentillä

Jeff Villari

Alan Froehlich

Dan Murphy

Heidi Hanson Hau

Emily A Stonehouse

Jeffrey D Keating

Chhayal Ghandi

Merja Penttilae

Shaunita Rose

Merja Penttila

Provided are a recombinant yeast producing 3-hydroxypropionic acid (3-HP) and a method for producing 3-HP using the same, more particularly, a recombinant yeast producing 3-HP, comprising an exogenous AADH gene; an endogenous or exogenous ACC gene; an exogenous MCR gene; and an exogenous HPDH gene, and producing 3-HP through [Pyruvate Acetaldehyde&#x2192;Acetyl-CoA Malonyl-CoA Malonate semialdehyde 3-HP] biosynthesis pathway, and a method for producing 3-HP using the same.

Recombinant yeast producing 3-hydroxypropionic acid and method for producing 3-hydroxypropionic acid using the same

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species, and fermentation processes using genetically modified yeast

The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.

Method for determining amounts of polynucleotide sequences present in cell or tissue samples

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Cells of the speciesand closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

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Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Genetically modified yeast species and fermentation processes using genetically modified yeast

The invention relates to a method for the production of xylitol, the method comprising The invention also relates to a microorganism, which has xylanolytic activity and has been genetically modified (i) to enhance its xylanolytic activity, and (ii) to reduce its xylitol metabolism.

Method for the production of xylitol

Shortened sophorose inducible promoters are provided. The invention further provides vectors containing such promoters hosts transformed with the same, and methods utilizing such promoters for the expression of operably linked sequences.

Promoters and uses thereof

Growing community of inventors

Helsinki, Finland

Marja Ilmen