Instituto De Salud Carlos Iii

Fundacion Publica Andaluza Progreso Y Salud

Marién Cobo Pulido

Karim Benabdellah El Khlanji

Pilar Muñoz Fernández

Francisco Martín Molina

Alejandra Gutierrez Guerrero

Karim Benabdellah

Miguel García Toscano

Per Anderson

Karim Benabdellah Elkhlanji

Ana Carrillo Gálves

The present invention solves the problem of providing more efficient barrier insulators to avoid vector silencing and to increase expression in the setting of gene transfer vectors, more particularly in the setting of gene transfer retroviral vectors. In this sense, the authors of the present invention have developed an improved insulator element, namely element IS2, which comprises the following combination of nucleic acid molecules, namely nucleic acid molecule HS4-650 bp as shown in SEQ ID No 2 and a synthetic S/MAR nucleic acid molecule containing 5 M/SARs recognition signatures (MRS) as shown in SEQ ID no 1.

Insulator to improve gene transfer vectors

Growing community of inventors

Seville, Spain

Marién Cobo Pulido